Overexpression of a Bacillus subilis amylase in E.coli and application in bread making

Bacterial alpha-amylases (EC3.2.1.1) have potential use in wide number of industrial applications such as textile, paper, detergent, food, fermentation and pharmaceutical industries. Recombinant DNA technology for amylase production involves the selection of an amylase gene, its insertion into an appropriate vector system, transformation in an efficient bacterial system to produce high amount of recombinant protein. In this context, the aim of this work is the overexpression of an α-amylase gene from Bacillus subtilis US572 in E.coli strain, the characterization of the recombinant enzyme and test the effect of different quantities added of amylase on wheat flours and bread characterization.