Human Bocaviruses (HBoV) were associated with respiratory diseases. Here, we assessed a TaqMan(®)-based PCR for the detection of all four HBoV subtype infections with a sensitivity up to 15 copies/reaction. To evaluate this assay on clinical samples, 178 nasopharyngeal aspirate specimens from pediatric cases were analyzed and HBoV genome was detected in 13 out of 178 patients with a viral load range between 1.6 × 10(3) and 9.4 × 10(7) copies/ml. These results indicated that this method could be used as an alternative technique for the diagnosis of HBoV infection.
Assessment of NS1 gene-specific real time quantitative TaqMan PCR for the detection of Human Bocavirus in respiratory samples
LIGOZZI, Marco;DIANI, ERICA;LISSANDRINI, FEDERICA;MAINARDI, ROSANNA;GIBELLINI, Davide
2017-01-01
Abstract
Human Bocaviruses (HBoV) were associated with respiratory diseases. Here, we assessed a TaqMan(®)-based PCR for the detection of all four HBoV subtype infections with a sensitivity up to 15 copies/reaction. To evaluate this assay on clinical samples, 178 nasopharyngeal aspirate specimens from pediatric cases were analyzed and HBoV genome was detected in 13 out of 178 patients with a viral load range between 1.6 × 10(3) and 9.4 × 10(7) copies/ml. These results indicated that this method could be used as an alternative technique for the diagnosis of HBoV infection.
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