BACKGROUND:The aims of this study were:i) to analyze and quantify the apolipoproteinCIIIglycoforms characterized by none, one or two sialic acids,(having different lipoprotein lipase-LPLinhibitoryactivity), ii) to assess their relationship with LPL activity and ApoA-V in CAD patients,iii) to analyze some previously identified plasma proteins in relation to lipids status.METHODS: ApoCIII glycoforms in four groups of patients (from “Verona Heart Study” biobank,)classified according to the total plasma concentration of ApoCIII and different triglyceride (TG)levels, were analyzed by a classical (isoelectric focusing/western blotting) and by a shotgun MSapproach. LPL activity (Fluorescent assay) and ApoA-V concentration (ELISA assay) weredetermined, and their correlations with lipid metabolism parameters were analyzed. We alsovalidated by 2D-WB some plasma proteins previously identified by 2-DE analysis.RESULTS The distribution of the ApoCIII glycoforms in the selected groups of patients are relatedto the TG levels, particularly the mono-sialylated isoform (ApoCIII-1) prevails in patients with thehighest TG levels. Moreover, investigating the relation among ApoA-V level, LPL activity and theother parameters of lipid metabolism, we obtained useful information on their interplay as antiatherogenicfactors. ApoA-V concentration was found within the normal range, however higherthan previously reported. LPL activity measured as Vmax did not show significant correlations withApoCIII . Finally 2D-WB showed peculiar proteomic patterns of several proteins associated withelevated apoCIII plasma concentrations .CONCLUSIONS: As compared with the other ones, mono – sialylated isoform of apo CIII ispreferentially associated with TG levels. Samples with elevated levels of apoCIII are characterizedby specific proteomic patterns.

ApoCIII glycoforms determination and proteomic analysis in plasma of coronary patients with different ApoCIII levels .

CHIARIELLO, CARMELA;CASTAGNA, Annalisa;CECCONI, Daniela;OLIVIERI, Oliviero
2015

Abstract

BACKGROUND:The aims of this study were:i) to analyze and quantify the apolipoproteinCIIIglycoforms characterized by none, one or two sialic acids,(having different lipoprotein lipase-LPLinhibitoryactivity), ii) to assess their relationship with LPL activity and ApoA-V in CAD patients,iii) to analyze some previously identified plasma proteins in relation to lipids status.METHODS: ApoCIII glycoforms in four groups of patients (from “Verona Heart Study” biobank,)classified according to the total plasma concentration of ApoCIII and different triglyceride (TG)levels, were analyzed by a classical (isoelectric focusing/western blotting) and by a shotgun MSapproach. LPL activity (Fluorescent assay) and ApoA-V concentration (ELISA assay) weredetermined, and their correlations with lipid metabolism parameters were analyzed. We alsovalidated by 2D-WB some plasma proteins previously identified by 2-DE analysis.RESULTS The distribution of the ApoCIII glycoforms in the selected groups of patients are relatedto the TG levels, particularly the mono-sialylated isoform (ApoCIII-1) prevails in patients with thehighest TG levels. Moreover, investigating the relation among ApoA-V level, LPL activity and theother parameters of lipid metabolism, we obtained useful information on their interplay as antiatherogenicfactors. ApoA-V concentration was found within the normal range, however higherthan previously reported. LPL activity measured as Vmax did not show significant correlations withApoCIII . Finally 2D-WB showed peculiar proteomic patterns of several proteins associated withelevated apoCIII plasma concentrations .CONCLUSIONS: As compared with the other ones, mono – sialylated isoform of apo CIII ispreferentially associated with TG levels. Samples with elevated levels of apoCIII are characterizedby specific proteomic patterns.
Cardio Artery Disease, Apolipoprotein, Lipoprtein Lipase
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/936494
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