Background. There is a need for non invasive and easily measurable biomarkers able to predict the severity of asthma. Aim. We sought to assess the association between indicators of asthma severity with peripheral blood eosinophils (B-EOS), fractional exaled nitric oxide level (FeNO) and urinary biomarkers of oxidative stress. Methods. All asthmatic subjects (375) recruited in the GEIRD study were considered. Each subject underwent an accurate clinical interview, lung function test (LF), and measurement of: FeNO (50, 100, 150, and 200 ml/s), B-EOS, and urinary concentrations (creatinine-standardized) of 8-OHdg and 8-isoprostane (ELISA assay). An asthma severity score (SS) was computed by a multiple correspondence analysis on the basis of self-reported asthma-like symptoms, hospitalization and number of asthma attacks reported in the past year. Spirometric FEV1% predicted and pre-brochodilator FEV1/FVC were used as indicators of lung function. To study the association of SS and LF indicators with the biomarkers, gamma regression and linear regression models were used respectively, stratifying by corticosteroids treatment. Results. The preliminary analysis showed that B-EOS was associated with SS and pre-brochodilator FEV1/FVC. Neutrophils were associated only with FEV1% predicted. FeNO200/250 was associated with SS and FEV1/FVC but not with FEV1% predicted. 8-isoprostane was associated with LF indicators.
Biomarkers of asthma severity in the gene environment interaction in respiratory disease (GEIRD) study
Calciano, Lucia;ACCORDINI, Simone;FERRARI, Marcello;Olivieri, Mario;Degan, Paolo;PASINI, Andrea;PASINI, Annalisa;ZANOLIN, Maria Elisabetta;DE MARCO, Roberto
2014-01-01
Abstract
Background. There is a need for non invasive and easily measurable biomarkers able to predict the severity of asthma. Aim. We sought to assess the association between indicators of asthma severity with peripheral blood eosinophils (B-EOS), fractional exaled nitric oxide level (FeNO) and urinary biomarkers of oxidative stress. Methods. All asthmatic subjects (375) recruited in the GEIRD study were considered. Each subject underwent an accurate clinical interview, lung function test (LF), and measurement of: FeNO (50, 100, 150, and 200 ml/s), B-EOS, and urinary concentrations (creatinine-standardized) of 8-OHdg and 8-isoprostane (ELISA assay). An asthma severity score (SS) was computed by a multiple correspondence analysis on the basis of self-reported asthma-like symptoms, hospitalization and number of asthma attacks reported in the past year. Spirometric FEV1% predicted and pre-brochodilator FEV1/FVC were used as indicators of lung function. To study the association of SS and LF indicators with the biomarkers, gamma regression and linear regression models were used respectively, stratifying by corticosteroids treatment. Results. The preliminary analysis showed that B-EOS was associated with SS and pre-brochodilator FEV1/FVC. Neutrophils were associated only with FEV1% predicted. FeNO200/250 was associated with SS and FEV1/FVC but not with FEV1% predicted. 8-isoprostane was associated with LF indicators.
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