CATALOGO DEI PRODOTTI DELLA RICERCA
IRIS è la soluzione IT che facilita la raccolta e la gestione dei dati relativi alle attività e ai prodotti della ricerca. Fornisce a ricercatori, amministratori e valutatori gli strumenti per monitorare i risultati della ricerca, aumentarne la visibilità e allocare in modo efficace le risorse disponibili.
EnglishWe investigated the effects of prostate carcinoma cells LNCaP on human DC maturation, superoxide anion and cytokine production. LNCaP promoted partial DC maturation and scarce IL-12 secretion. The dectin 1 agonist glucan, an immuno-stimulating agent able to induce DC maturation but not IL-12 production, increased IL-12 release by DC cultured with LNCaP. Interestingly, LNCaP decreased superoxide anion production, but enhanced IL-1β, IL-23, IL-6 and TNF-α secretion in glucan-stimulated DC. Conversely LNCaP did not affect IL-1β, IL-23 and TNF-α, but inhibited IL-12 and IL-6 secretion induced by Toll-like receptors agonists LPS and Pam3CSK4, that elicit DC maturation but not superoxide anion generation. The NADPH oxidase inhibitor DPI and the superoxide anion scavenger SOD increased dectin 1-dependent IL-1β, IL-23, IL-6 and TNF-α release by DC, reproducing the effect of LNCaP, but did not affect IL-12 secretion by glucan-stimulated DC and cytokine release in LPS- or Pam3CSK4-treated DC both in presence or absence of LNCaP. Conditioned media of DC co-cultured with LNCaP activated IFN-γ production by natural killer cells and modulated IFN-γ and IL-17 release by CD4+ lymphocytes. Experiments performed with an IL-12-blocking antibody demonstrated that these effects arise from LNCaP- and glucan-dependent regulation of IL-12 production by DC. Our results indicate that LNCaP modulate DC cytokine release through NADPH oxidase-related or NADPH oxidase-unrelated mechanisms, depending on the type of cytokine secreted and/or receptor engaged when DC interact with tumor cells. The LNCaP-elicited IL-12 production by DC is enhanced by glucan and deeply influences the activity of natural killer cells and CD4+ lymphocytes.
| Titolo: | Prostate carcinoma cells LNCaP differentially regulate cytokine release in dectin 1- and Toll-like receptor- stimulated human monocyte-derived dendritic cells | |
| Autori: | ||
| Data di pubblicazione: | 2011 | |
| Abstract: | We investigated the effects of prostate carcinoma cells LNCaP on human DC maturation, superoxide anion and cytokine production. LNCaP promoted partial DC maturation and scarce IL-12 secretion. The dectin 1 agonist glucan, an immuno-stimulating agent able to induce DC maturation but not IL-12 production, increased IL-12 release by DC cultured with LNCaP. Interestingly, LNCaP decreased superoxide anion production, but enhanced IL-1β, IL-23, IL-6 and TNF-α secretion in glucan-stimulated DC. Conversely LNCaP did not affect IL-1β, IL-23 and TNF-α, but inhibited IL-12 and IL-6 secretion induced by Toll-like receptors agonists LPS and Pam3CSK4, that elicit DC maturation but not superoxide anion generation. The NADPH oxidase inhibitor DPI and the superoxide anion scavenger SOD increased dectin 1-dependent IL-1β, IL-23, IL-6 and TNF-α release by DC, reproducing the effect of LNCaP, but did not affect IL-12 secretion by glucan-stimulated DC and cytokine release in LPS- or Pam3CSK4-treated DC both in presence or absence of LNCaP. Conditioned media of DC co-cultured with LNCaP activated IFN-γ production by natural killer cells and modulated IFN-γ and IL-17 release by CD4+ lymphocytes. Experiments performed with an IL-12-blocking antibody demonstrated that these effects arise from LNCaP- and glucan-dependent regulation of IL-12 production by DC. Our results indicate that LNCaP modulate DC cytokine release through NADPH oxidase-related or NADPH oxidase-unrelated mechanisms, depending on the type of cytokine secreted and/or receptor engaged when DC interact with tumor cells. The LNCaP-elicited IL-12 production by DC is enhanced by glucan and deeply influences the activity of natural killer cells and CD4+ lymphocytes. | |
| Handle: | http://hdl.handle.net/11562/804164 | |
| Appare nelle tipologie: | 04.03 Poster |
File in questo prodotto:
Copyright © 2022