Lo5 line responded to NO3 -‐ treatment (induction) faster than T250 line at each concentration assayed. Even if the two inbred lines reached highest induction at different times (2 h for 500 μM and 4 h for 200 μM for Lo5 and 8 h for 500 μM and 12 h for 200 μM for T250), they showed similar values of NO3 -‐ uptake rate. On the basis of this different behaviour, roots were sampled at different times (1, 2 and 4 h for Lo5 and 5, 11 and 12 h for T250) after NO3 -‐ treatment (200 μM) in order to perform a “genome-‐wide” transcriptional characterization. Data analysis of microarray experiments, performed with a chip that allows monitoring the expression of about 60000 maize transcripts, showed that roots of Lo5 and T250 inbred lines responded to different periods of NO3 -‐ contact through a modulation of a different number of transcripts. Changes in root Lo5 transcriptome involved a higher number of transcripts (about 5000 at 2 h) relative to T250 (about 1900 at 12 h). After the peak, transcripts modulated by the treatment in Lo5 decreased, with the behaviour different from T250 where the number increased linearly up to 12 h. In addition, NO3 -‐ treatment affected the Lo5 and T250 Gene Ontology (GO) terms in a different way. PAGE analysis performed in Lo5 suggests that processes related to protein modifications (i.e. protein phosphorylation) and translation were positively affected at 1 h and 4 h, passing through a negative phase of regulation at 2 h. On the contrary, “protein metabolic process” terms were down-‐regulated at all sampling time points in T250 relative to Lo5 and only in T250 was a positive modulation of transcripts related to nitrogen metabolism (“nitrogen compound of metabolic process”) recorded at 5 and 11 h. Furthermore, in the two lines only about 400 transcripts were modulated in common in response to NO3 -‐.
Comparison of changes in root transcriptome of two maize inbred lines in response to nitrate treatment
ZAMBONI, Anita;PII, Youry;VARANINI, Zeno
2013-01-01
Abstract
Lo5 line responded to NO3 -‐ treatment (induction) faster than T250 line at each concentration assayed. Even if the two inbred lines reached highest induction at different times (2 h for 500 μM and 4 h for 200 μM for Lo5 and 8 h for 500 μM and 12 h for 200 μM for T250), they showed similar values of NO3 -‐ uptake rate. On the basis of this different behaviour, roots were sampled at different times (1, 2 and 4 h for Lo5 and 5, 11 and 12 h for T250) after NO3 -‐ treatment (200 μM) in order to perform a “genome-‐wide” transcriptional characterization. Data analysis of microarray experiments, performed with a chip that allows monitoring the expression of about 60000 maize transcripts, showed that roots of Lo5 and T250 inbred lines responded to different periods of NO3 -‐ contact through a modulation of a different number of transcripts. Changes in root Lo5 transcriptome involved a higher number of transcripts (about 5000 at 2 h) relative to T250 (about 1900 at 12 h). After the peak, transcripts modulated by the treatment in Lo5 decreased, with the behaviour different from T250 where the number increased linearly up to 12 h. In addition, NO3 -‐ treatment affected the Lo5 and T250 Gene Ontology (GO) terms in a different way. PAGE analysis performed in Lo5 suggests that processes related to protein modifications (i.e. protein phosphorylation) and translation were positively affected at 1 h and 4 h, passing through a negative phase of regulation at 2 h. On the contrary, “protein metabolic process” terms were down-‐regulated at all sampling time points in T250 relative to Lo5 and only in T250 was a positive modulation of transcripts related to nitrogen metabolism (“nitrogen compound of metabolic process”) recorded at 5 and 11 h. Furthermore, in the two lines only about 400 transcripts were modulated in common in response to NO3 -‐.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
