One of the major pollution problems within the industrialized countries is soil contamination caused by spills of petroleum hydrocarbons, included diesel range organic (DRO) compounds. Bioremediation of hydrocarbon-polluted soils, which exploits the ability of microorganisms to degrade and/or detoxify organic contaminants, has been established as an efficient, economic, and environmentally sound treatment. However, knowledge on microbial composition during clean-up processes is very critical, especially in determining whether sufficient potential is available for bioelimination to occur. In the present study, DGGE analysis was carried out for the characterisation of the bacterial community of a soil contaminated by 580.0 mg\kg diesel fuel. This kind of approach evidenced that structure and diversity of the bacterial community typical of the pristine (uncontaminated) soil was recovered after landfarming treatment. Moreover, in order to prepare an inoculum able to degrade DRO compounds, 103 bacterial strains enriched in the presence of diesel fuel were isolated and characterised. Acinetobacter calcoaceticus and A. junii resulted the most representative species in the soil considered.
Study of the bacterial community in a diesel-fuel polluted soil undergoing bio-reclamation through landfarming treatment
VALLINI, Giovanni
2006-01-01
Abstract
One of the major pollution problems within the industrialized countries is soil contamination caused by spills of petroleum hydrocarbons, included diesel range organic (DRO) compounds. Bioremediation of hydrocarbon-polluted soils, which exploits the ability of microorganisms to degrade and/or detoxify organic contaminants, has been established as an efficient, economic, and environmentally sound treatment. However, knowledge on microbial composition during clean-up processes is very critical, especially in determining whether sufficient potential is available for bioelimination to occur. In the present study, DGGE analysis was carried out for the characterisation of the bacterial community of a soil contaminated by 580.0 mg\kg diesel fuel. This kind of approach evidenced that structure and diversity of the bacterial community typical of the pristine (uncontaminated) soil was recovered after landfarming treatment. Moreover, in order to prepare an inoculum able to degrade DRO compounds, 103 bacterial strains enriched in the presence of diesel fuel were isolated and characterised. Acinetobacter calcoaceticus and A. junii resulted the most representative species in the soil considered.
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