Establishment of functional genomics in Vitis ssp. is still challenging, due to the lack of reliable high-throughput tools for grapevine transformation. Virus-induced gene silencing (VIGS) strategy makes use of a plant virus-based vector carrying a sequence of an endogenous plant gene. By delivery of the vector and replication of the recombinant virus within the plant, the plant defense mechanism is activated against the virus resulting in silencing of the plant gene. This system has been recently applied in grapevine using Grapevine Virus A (GVA) and Grapevine Leafroll-Associated Virus-2 (GLRaV-2) –based vectors (1, 2). We are developing a new VIGS system using a vector based on Grapevine Algerian Latent Virus (GALV).
DEVELOPMENT OF A NEW VIGS VECTOR FOR GRAPEVINE, BASED ON GRAPEVINE ALGERIAN LATENT VIRUS.
LOVATO, Arianna;POLVERARI, Annalisa
2013-01-01
Abstract
Establishment of functional genomics in Vitis ssp. is still challenging, due to the lack of reliable high-throughput tools for grapevine transformation. Virus-induced gene silencing (VIGS) strategy makes use of a plant virus-based vector carrying a sequence of an endogenous plant gene. By delivery of the vector and replication of the recombinant virus within the plant, the plant defense mechanism is activated against the virus resulting in silencing of the plant gene. This system has been recently applied in grapevine using Grapevine Virus A (GVA) and Grapevine Leafroll-Associated Virus-2 (GLRaV-2) –based vectors (1, 2). We are developing a new VIGS system using a vector based on Grapevine Algerian Latent Virus (GALV).
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