Phenotypic plasticity refers to the range of phenotypes a single genotype can express as a function of its environment. We investigated phenotypic plasticity in grapevine by comparing the berry transcriptome in a single clone of the vegetatively-propagated common grapevine species Vitis vinifera (cv Corvina) through three consecutive vintages cultivated in 11 different vineyards in the Verona area of Italy.Most of the berry transcriptome clustered by vintage rather than common environmental conditions or viticulture practices, and transcripts related to secondary metabolism showed high sensitivity towards different climates, as confirmed also by metabolomic data obtained from the same samples. When we compared 11 vineyards during one harvesting season, we found that the environmentally-sensitive berry transcriptome comprised 5% of all protein-coding genes and 18% of transcripts modulated during berry development. Specific plastic transcripts were associated mainly with transcription factors, translation, transport, secondary metabolism and stress boxes, and plastic transcriptome reprogramming was more intense in the vintage characterized by extreme weather conditions.We also identified a set of genes that lacked plasticity, showing either constitutive expression or similar modulation in all berries. Our data provide the first step towards the characterization of grapevine transcriptome plasticity under different agricultural systems.
The plasticity of grapevine berry transcriptome
PEZZOTTI, Mario;DAL SANTO, SILVIA;TORNIELLI, Giovanni Battista;ZENONI, Sara;FASOLI, Marianna;ANESI, Andrea;GUZZO, Flavia;DELLEDONNE, Massimo
2013-01-01
Abstract
Phenotypic plasticity refers to the range of phenotypes a single genotype can express as a function of its environment. We investigated phenotypic plasticity in grapevine by comparing the berry transcriptome in a single clone of the vegetatively-propagated common grapevine species Vitis vinifera (cv Corvina) through three consecutive vintages cultivated in 11 different vineyards in the Verona area of Italy.Most of the berry transcriptome clustered by vintage rather than common environmental conditions or viticulture practices, and transcripts related to secondary metabolism showed high sensitivity towards different climates, as confirmed also by metabolomic data obtained from the same samples. When we compared 11 vineyards during one harvesting season, we found that the environmentally-sensitive berry transcriptome comprised 5% of all protein-coding genes and 18% of transcripts modulated during berry development. Specific plastic transcripts were associated mainly with transcription factors, translation, transport, secondary metabolism and stress boxes, and plastic transcriptome reprogramming was more intense in the vintage characterized by extreme weather conditions.We also identified a set of genes that lacked plasticity, showing either constitutive expression or similar modulation in all berries. Our data provide the first step towards the characterization of grapevine transcriptome plasticity under different agricultural systems.
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