Contamination, degradation or unsuitable storage conditions of biological samples may produce partial genetic profiles and amplification artifacts of difficult interpretation. Allele drop-out is one of the most frequent stochastic artifacts involving degraded DNA. We report a case where a woman tested positive for alcohol tests performed on blood samples taken following the police custody, had doubts about the origin of the biological sample (blood) analyzed. After a few weeks she has required the comparison of its genetic profile obtained from a saliva sample with that obtained from the blood sample that had been preserved. A stain of blood sample was placed on paper and was divided into four parts, one of these was immediately analyzed, providing a genetic profile that was not compatible with the genetic profile of the woman. Subsequently, another part of the bloodstain was underwent to genetic analysis providing a different genetic profile than the previous stain, but fully compatible with that of the woman. Unexpectedly, two portions of the same trace have produced two different profiles.

Two different genetic profiles from a bloodstain: which one is true?

TURRINA, Stefania;FILIPPINI, Giulia;DE LEO, Domenico
2012

Abstract

Contamination, degradation or unsuitable storage conditions of biological samples may produce partial genetic profiles and amplification artifacts of difficult interpretation. Allele drop-out is one of the most frequent stochastic artifacts involving degraded DNA. We report a case where a woman tested positive for alcohol tests performed on blood samples taken following the police custody, had doubts about the origin of the biological sample (blood) analyzed. After a few weeks she has required the comparison of its genetic profile obtained from a saliva sample with that obtained from the blood sample that had been preserved. A stain of blood sample was placed on paper and was divided into four parts, one of these was immediately analyzed, providing a genetic profile that was not compatible with the genetic profile of the woman. Subsequently, another part of the bloodstain was underwent to genetic analysis providing a different genetic profile than the previous stain, but fully compatible with that of the woman. Unexpectedly, two portions of the same trace have produced two different profiles.
Bloodstain; Genetic profile; STRs
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11562/454541
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