Tomato Aucsia genes have been shown to control fruit initiation and affect auxin-related processes. The Aucsia homologues of Arabidopsis, AtAucsia-1 and AtAucsia-2 are expressed in all plant organs and preferentially in inflorescences. Reporter gene studies have been performed with either Aucsia-1p:GUS or with Aucsia-2p:GUS to visualize the expression pattern of the two genes during seedlings and plant development. Furthermore, we have analysed two Arabidopsis Salk lines: SALK_117986 a T-DNA insertion AtAucsia-1 mutant and SALK_065659 a T-DNA insertion AtAucsia-2 mutant. In plants homozygous for the insertion in AtAucsia-2, AtAucsia-2 mRNA level is 50% lower than in wild-type. Homozygous ataucsia-1 mutant shows no detectable expression of AtAucsia-1 mRNA. Homozygous ataucsia-1 mutant seedlings show no visible differences in growth compared with wild-type, but when cultivated with growth-inhibiting concentrations of IAA, they are less sensitive indicating that ataucsia-1 mutants might be affected in auxin response. Homozygous ataucsia-2 mutant seedlings display a slight reduction in primary root growth compared to wild-type, whereas their sensitivity to auxin is not altered.
Analysis of Arabidopsis thaliana Aucsia mutants
MOLESINI, Barbara;PII, Youry;PANDOLFINI, Tiziana;SPENA, Angelo
2009-01-01
Abstract
Tomato Aucsia genes have been shown to control fruit initiation and affect auxin-related processes. The Aucsia homologues of Arabidopsis, AtAucsia-1 and AtAucsia-2 are expressed in all plant organs and preferentially in inflorescences. Reporter gene studies have been performed with either Aucsia-1p:GUS or with Aucsia-2p:GUS to visualize the expression pattern of the two genes during seedlings and plant development. Furthermore, we have analysed two Arabidopsis Salk lines: SALK_117986 a T-DNA insertion AtAucsia-1 mutant and SALK_065659 a T-DNA insertion AtAucsia-2 mutant. In plants homozygous for the insertion in AtAucsia-2, AtAucsia-2 mRNA level is 50% lower than in wild-type. Homozygous ataucsia-1 mutant shows no detectable expression of AtAucsia-1 mRNA. Homozygous ataucsia-1 mutant seedlings show no visible differences in growth compared with wild-type, but when cultivated with growth-inhibiting concentrations of IAA, they are less sensitive indicating that ataucsia-1 mutants might be affected in auxin response. Homozygous ataucsia-2 mutant seedlings display a slight reduction in primary root growth compared to wild-type, whereas their sensitivity to auxin is not altered.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
