Resveratrol activity on MCL cell line jeko-1: a proteomic approach

Background Very few studies have attempted the investigation of mantle cell lymphoma (MCL) by proteomic tools. We studied MCL cell lines using the PhosphoScan approach. We then investigated the importance of the identified pathways for the survival of MCL cell lines using a functional validation approach.Results 421 peptides were identified, corresponding to 341 proteins. The most represented pathways suggest the tonic activation of the B-cell receptor signalling. Inhibition of the upstream molecule SYK by Piceatannol, followed by the reduction of downstream target phosphorylation, caused apoptosis of MCL cells. Immunofluorescence studies showed a compartment transition of P-SYK after inhibition.