Purpose: To compare T1- or T2-weighted magnetic resonanceimaging (MRI) and ultrasonography (US) as tools forin vivo mapping of different tissue components in spontaneoustumors of transgenic mice.Materials and Methods: Human-like mammary adenocarcinomasfrom FVB/neuT transgenic mice were analyzed byT2-weighted and T1-weighted MRI at 4.7 Tesla and US andthen, after excision, were paraffin-embedded for histologicanalysis. The histologic samples were prepared taking careto obtain sections that spatially matched the MRI and USimages as precisely as possible.Results: US can obtain basic information such as the sizeof developing tumors in experimental animals and canidentify necrotic areas. T2-weighted MRI, especially if comparedto T1-weighted MRI and/or US, allows advancedanalysis of morphologic aspects, with high resolution in thedifferentiation of details of necrotic areas such as coagulation,liquefaction, biphasic splitting of cysts, and fibroticand lipidic infiltration.Conclusion: Of the three methods, T2-weighted MRI providesthe most information about the anatomy of tumors.However, when distinctions between the different types ofnecrosis are not needed, US analysis is to be preferred forits practicality.
In vivo mapping of histological and vascular aspect of mammary tumors in transgenic mice using MRI and ultrasonography
GALIE', Mirco;D'ONOFRIO, Mirko;CALDERAN, Laura;NICOLATO, Elena;MARZOLA, Pasquina;SBARBATI, Andrea
2004
Abstract
Purpose: To compare T1- or T2-weighted magnetic resonanceimaging (MRI) and ultrasonography (US) as tools forin vivo mapping of different tissue components in spontaneoustumors of transgenic mice.Materials and Methods: Human-like mammary adenocarcinomasfrom FVB/neuT transgenic mice were analyzed byT2-weighted and T1-weighted MRI at 4.7 Tesla and US andthen, after excision, were paraffin-embedded for histologicanalysis. The histologic samples were prepared taking careto obtain sections that spatially matched the MRI and USimages as precisely as possible.Results: US can obtain basic information such as the sizeof developing tumors in experimental animals and canidentify necrotic areas. T2-weighted MRI, especially if comparedto T1-weighted MRI and/or US, allows advancedanalysis of morphologic aspects, with high resolution in thedifferentiation of details of necrotic areas such as coagulation,liquefaction, biphasic splitting of cysts, and fibroticand lipidic infiltration.Conclusion: Of the three methods, T2-weighted MRI providesthe most information about the anatomy of tumors.However, when distinctions between the different types ofnecrosis are not needed, US analysis is to be preferred forits practicality.
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