These preliminary findings show that the success rate of immature ooplasts reconstituted with large cells is significantly higher than that employing small cumulus cells; nevertheless, there was no difference between these groups in the ability to extrude a polar body. Moreover, although the overall yield of the reconstituted haploid oocytes was rather low, their karyotypes indicate that this approach can provide normal haploid oocytes. Since the majority of the transplanted somatic cells were presumably electrofused at G0/G1, it is possible that the efficiency of this technique may be improved by more careful synchronization of the cell cycle before transfer.
The efficiency of "production" of mammalian haploid oocytes as a function of the cell cycle stage of the transferred somatic karyoplasts
RAFFAELLI, Ricciarda;
2000-01-01
Abstract
These preliminary findings show that the success rate of immature ooplasts reconstituted with large cells is significantly higher than that employing small cumulus cells; nevertheless, there was no difference between these groups in the ability to extrude a polar body. Moreover, although the overall yield of the reconstituted haploid oocytes was rather low, their karyotypes indicate that this approach can provide normal haploid oocytes. Since the majority of the transplanted somatic cells were presumably electrofused at G0/G1, it is possible that the efficiency of this technique may be improved by more careful synchronization of the cell cycle before transfer.
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