Vanillin-(6'-0-galloy1)-beta-glucopyranoside (VGG), 3,4,5-trimethoxyphenyl-(6'-0-galloy1)-beta-glucopyranoside (TMPGG), and (6R,9R)-3-oxo-alpha-iono1-9-0-(6'-0-galloy1)-beta-glucopyranoside (macarangioside E) were identified as aroma precursors in oak wood.An LC-MS/MS method was developed and validated to quantify these three galloylglucoside compounds in brandies aged in oak barrels. The detection system consisted of a triple quadrupole mass analyser operating in multiple reaction monitoring (MRM) mode. For the first time, vanillin-beta-Dxylopyranoside (VX) was synthesised for use as an internal standard. The detection limits (48 p,g L-1 for VGG, 52 mu g L-1 for TMPGG, and 19 mu g L-1 for macarangioside E) were low enough to quantify these aroma precursors in spirits without any sample preparation. (C) 2016 Elsevier B.V. All rights reserved.

Quantification of three galloylglucoside flavour precursors by liquid chromatography tandem mass spectrometry in brandies aged in oak wood barrels

Davide Slaghenaufi
;
2016-01-01

Abstract

Vanillin-(6'-0-galloy1)-beta-glucopyranoside (VGG), 3,4,5-trimethoxyphenyl-(6'-0-galloy1)-beta-glucopyranoside (TMPGG), and (6R,9R)-3-oxo-alpha-iono1-9-0-(6'-0-galloy1)-beta-glucopyranoside (macarangioside E) were identified as aroma precursors in oak wood.An LC-MS/MS method was developed and validated to quantify these three galloylglucoside compounds in brandies aged in oak barrels. The detection system consisted of a triple quadrupole mass analyser operating in multiple reaction monitoring (MRM) mode. For the first time, vanillin-beta-Dxylopyranoside (VX) was synthesised for use as an internal standard. The detection limits (48 p,g L-1 for VGG, 52 mu g L-1 for TMPGG, and 19 mu g L-1 for macarangioside E) were low enough to quantify these aroma precursors in spirits without any sample preparation. (C) 2016 Elsevier B.V. All rights reserved.
2016
Aroma precursor
Brandy
Galloylglucoside
LC-QqQ-MS
Multiple reaction monitoring
Alcoholic Beverages
Flavoring Agents
Food Analysis
Humans
Limit of Detection
Chromatography, Liquid
Tandem Mass Spectrometry
Wood
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/1038580
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