We evaluated the impact of a number of clarifying agents on the concentration of S-3- (hexan-1-ol)-L-cysteine (Cys-3SH) and S-3-(hexan-1-ol)-L-glutathione (GSH-3SH). 19 clear grape juices were spiked with a grape skin tannin rich in Cys-3SH and GSH-3SH. Juices were then treated with Na-bentonite, PVPP or charcoal (1 g/L) and cold settled. The concentration of precursors was measured and compared to the corresponding untreated control juices in the devatted samples. Cys-3SH and GSH-3SH were analysed using UHPLC-MS/MS and accuracy was guaranteed with deuterated internal standards. Only charcoal caused a statistically significant depletion of both precursors, quantitatively limited even at the highest dose adopted. Technologically, the clarifiers used in juice affected the thiol precursors in a marginal manner

Clarifying agents and 3-sulfanylhexanol precursors in grape juice

Slaghenaufi, D.;
2016-01-01

Abstract

We evaluated the impact of a number of clarifying agents on the concentration of S-3- (hexan-1-ol)-L-cysteine (Cys-3SH) and S-3-(hexan-1-ol)-L-glutathione (GSH-3SH). 19 clear grape juices were spiked with a grape skin tannin rich in Cys-3SH and GSH-3SH. Juices were then treated with Na-bentonite, PVPP or charcoal (1 g/L) and cold settled. The concentration of precursors was measured and compared to the corresponding untreated control juices in the devatted samples. Cys-3SH and GSH-3SH were analysed using UHPLC-MS/MS and accuracy was guaranteed with deuterated internal standards. Only charcoal caused a statistically significant depletion of both precursors, quantitatively limited even at the highest dose adopted. Technologically, the clarifiers used in juice affected the thiol precursors in a marginal manner
bentonite, charcoal, grape juice, polyvinylpolypyrrolidone, varietal thiols
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/1038571
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