Clustered regularly interspaced short palindromic repeats (CRISPR) technologies allow for facile genomic modification in a site-specific manner. A key step in this process is the in silico design of single guide RNAs to efficiently and specifically target a site of interest. To this end, it is necessary to enumerate all potential off-target sites within a given genome that could be inadvertently altered by nuclease-mediated cleavage. Currently available software for this task is limited by computational efficiency, variant support or annotation, and assessment of the functional impact of potential off-target effects.

CRISPRitz: rapid, high-throughput and variant-aware in silico off-target site identification for CRISPR genome editing

Samuele Cancellieri;Nicola Bombieri;Rosalba Giugno;Luca Pinello
2020

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) technologies allow for facile genomic modification in a site-specific manner. A key step in this process is the in silico design of single guide RNAs to efficiently and specifically target a site of interest. To this end, it is necessary to enumerate all potential off-target sites within a given genome that could be inadvertently altered by nuclease-mediated cleavage. Currently available software for this task is limited by computational efficiency, variant support or annotation, and assessment of the functional impact of potential off-target effects.
CRISPR,genome editing, genetic, bioinformatics
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/1023826
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