Evaluation of the phenolic profile and immunoreactivity of Mal d 3 allergen in ancient apple cultivars from Italy

peared from orchards in Italy. Some of these ancient apple cultivars often possess high nutraceutical values and display lower allergenicity than the modern ones, supporting the so-called ‘green revolution’ theory. RESULTS: In this study, the phenolic composition and the antioxidant activity of five ancient apple cultivars (‘Belfiore’, ‘Pomella Genovese’, ‘Gravenstein’, ‘Bella del Bosco’, and ‘Piatlin’) were compared with a ‘Golden Delicious’ commercial cultivar. Additionally, apples were tested for their potential allergenicity by detecting the presence of Mal d 3, a non-specific lipid transfer protein that represents the main apples' allergen. All apples came from northern Italy (Trentino Region) and were organically produced. Results showed that, for all cultivars, the skins contained more polyphenols than the pulps. ‘Bella del Bosco’ had the highest amount of polyphenols and antioxidant activity, whereas ‘Piatlin’ had the lowest phenolic content. All ancient cultivars presented a higher amount of pulp phenolic compounds than ‘Golden Delicious’. Immunoblotting techniques showed that ‘Bella del Bosco’ and ‘Piatlin’ had very low quantities of Mal d 3 allergen; hence, they can be considered hypoallergenic cultivars. CONCLUSIONS: The preservation of ancient apple cultivars would be of great importance, not only to maintain the biodiversity but also for their nutritional properties. The hypoallergenic activity of some of these cultivars could be of interest also for the preparation of different apple-based products.