To shed light on the of MtN5 during early symbiotic events, we investigated the metabolic pathways for lipid production associated with the MtN5overespression. We carried out a comparative transcriptomic analysis of MtN5overexpressing (MtN5ox) and wild type roots after 72h of Sinorhizobium meliloti inoculation; in the same experimental conditions, we assayed the effects of MtN5 overexpression on the root lipid profile. In MtN5ox roots, we identified a quite relevant number of differential expressed genes involved in lipid transport and metabolism. The major changes were detected in genes implicated in phopholipid signalling pathways such as myo-inositol 1-phosphate synthases, phosphatidylinositolspecific phospholipase, phosphatidylinositol kinases, phosphatidylinositol transfer protein, inositol mono-phosphatase. All these transcripts were down-regulated in inoculated MtN5ox roots. Interestingly we demonstrated that MtN5 functioning is dependent on phospholipase D but not on phospholipase C activity (Pii et al., 2012). The analysis of the root lipid fraction revealed that MtN5 overexpressing roots displayed a general increase in the lipid content, that was especially relevant for galactolipids that are component of plastid and symbiosome membranes.
MEDICAGO TRUNCATULA ROOTS OVEREXPRESSING THE NSLTP N5 DISPLAY MODIFICATIONS IN THE LIPID PROFILE AND CHANGES IN THE EXPRESSION OF GENES INVOLVED IN LIPID METABOLISM AFTER SINORHIZOBIUM MELILOTI INFECTION
SANTI C.;MOLESINI B.;GUZZO F.;PANDOLFINI T.
2017-01-01
Abstract
To shed light on the of MtN5 during early symbiotic events, we investigated the metabolic pathways for lipid production associated with the MtN5overespression. We carried out a comparative transcriptomic analysis of MtN5overexpressing (MtN5ox) and wild type roots after 72h of Sinorhizobium meliloti inoculation; in the same experimental conditions, we assayed the effects of MtN5 overexpression on the root lipid profile. In MtN5ox roots, we identified a quite relevant number of differential expressed genes involved in lipid transport and metabolism. The major changes were detected in genes implicated in phopholipid signalling pathways such as myo-inositol 1-phosphate synthases, phosphatidylinositolspecific phospholipase, phosphatidylinositol kinases, phosphatidylinositol transfer protein, inositol mono-phosphatase. All these transcripts were down-regulated in inoculated MtN5ox roots. Interestingly we demonstrated that MtN5 functioning is dependent on phospholipase D but not on phospholipase C activity (Pii et al., 2012). The analysis of the root lipid fraction revealed that MtN5 overexpressing roots displayed a general increase in the lipid content, that was especially relevant for galactolipids that are component of plastid and symbiosome membranes.
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