Among the wide range of nanoparticles (NPs) studied for diagnostic and thera-peutic applications lanthanide-doped nanosystems have raised special interest [1]. Their very small dimension (10 nm) and upconversion emission property have increased the range of their applications from contrast agent probes in bioimaging to drug delivery systems [2,3]. Here, the cytotoxicity of rare earth (Yb and Er)-doped CaF2 and SrF2 NPs has been investigated both in vitro and in vivo. In vitro studies have been conducted in a motoneuron cell line as model of neuronal interaction, and in a line of human dendritic cells which play a key role in the immune response. In the motoneuron cell line, a weak response was observed at early time points while the cell viability showed an increment, except for the highest concentration of lan-thanide-doped NPs. The levels of cytokines released from human dendritic cells were low and dose-dependent. The NP biodistribution was investigated after a sin-gle peripheral administration in mice. Aggregates of NPs were shown, with differ-ent techniques, mostly in peripheral organs (spleen and liver) after one day. A limited penetration of both CaF2 and SrF2 NPs was seen in the brain parenchyma, associated with a mild astrocytic activation. Since the present in vitro findings indicate that lan-thanide-doped NPs are safe, and the in vivo data show that they can enter the brain parenchyma crossing the blood-brain barrier, these NPs may represent promising tools for diagnostic and therapeutical applications Lanthanide-doped CaF2 and SrF2 nanoparticles for biomedical applications: in vivo and in vitro experimental studies |

Lanthanide-doped CaF2 and SrF2 nanoparticles for biomedical applications: in vivo and in vitro experimental studies

Corinne Portioli
Writing – Original Draft Preparation
;
Marco Pedroni
Formal Analysis
;
Donatella Benati
Formal Analysis
;
Stefano Dusi;Marta Donini
Formal Analysis
;
Raffaella Mariotti;Roberta Bonafede;Luigi Perbellini
Supervision
;
Marzia Cerpelloni
Formal Analysis
;
Adolfo Speghini
Writing – Review & Editing
;
Marina Bentivoglio
Writing – Review & Editing
2015

Abstract

Among the wide range of nanoparticles (NPs) studied for diagnostic and thera-peutic applications lanthanide-doped nanosystems have raised special interest [1]. Their very small dimension (10 nm) and upconversion emission property have increased the range of their applications from contrast agent probes in bioimaging to drug delivery systems [2,3]. Here, the cytotoxicity of rare earth (Yb and Er)-doped CaF2 and SrF2 NPs has been investigated both in vitro and in vivo. In vitro studies have been conducted in a motoneuron cell line as model of neuronal interaction, and in a line of human dendritic cells which play a key role in the immune response. In the motoneuron cell line, a weak response was observed at early time points while the cell viability showed an increment, except for the highest concentration of lan-thanide-doped NPs. The levels of cytokines released from human dendritic cells were low and dose-dependent. The NP biodistribution was investigated after a sin-gle peripheral administration in mice. Aggregates of NPs were shown, with differ-ent techniques, mostly in peripheral organs (spleen and liver) after one day. A limited penetration of both CaF2 and SrF2 NPs was seen in the brain parenchyma, associated with a mild astrocytic activation. Since the present in vitro findings indicate that lan-thanide-doped NPs are safe, and the in vivo data show that they can enter the brain parenchyma crossing the blood-brain barrier, these NPs may represent promising tools for diagnostic and therapeutical applications Lanthanide-doped CaF2 and SrF2 nanoparticles for biomedical applications: in vivo and in vitro experimental studies |
Lanthanide ions; Nanoparticles; Cell viability; Biodistribution.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/985215
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