the recent paper by Appel et al., evaluated the use of the basophil activaion test (BAT) in sesame allergy and concluded that BAT should be preferentially used in association with high protein sesame extract-skin prick test (HSPE-SPT) for the diagnosis of sesame food allergy, so preventing any ethical issue regarding the possble risk associated with oral food challenge (OFC)1 . The authors used a non commercial BAT approach endowed with the CD123pos /HLADRneg phenotyping protocol and using CD63 and/or CD203c as activation markers2,3 . Basophils were initially captured in flow cytometry (FC) from a not clearly separated side scatter (SSC)-CD123pos . This article is protected by copyright. All rights reserved.
On the reliability of the CD123-endowed basophil activation test (BAT) and its application in food allergy
Chirumbolo, Salvatore
;Vella, Antonio
2018-01-01
Abstract
the recent paper by Appel et al., evaluated the use of the basophil activaion test (BAT) in sesame allergy and concluded that BAT should be preferentially used in association with high protein sesame extract-skin prick test (HSPE-SPT) for the diagnosis of sesame food allergy, so preventing any ethical issue regarding the possble risk associated with oral food challenge (OFC)1 . The authors used a non commercial BAT approach endowed with the CD123pos /HLADRneg phenotyping protocol and using CD63 and/or CD203c as activation markers2,3 . Basophils were initially captured in flow cytometry (FC) from a not clearly separated side scatter (SSC)-CD123pos . This article is protected by copyright. All rights reserved.
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