FTIR microspectroscopy was used to observe changes in the inner and outer skin sides during the ripening process in cultivar Corvina. Measurements were performed in ATR mode without any sample preparation to preserve the native cell wall structure, with the aim of providing information about the in-muro organization of the cell wall polymers and functional groups. Absorption spectra were analyzed by an automated spectra selection procedure, Principal Component Analysis and graphical heatmap representation of the data, choosing two distinct normalizations for the two skin sides, in order to unravel modifications masked by their distinctive traits. Genome-wide transcriptomic approach was used to profile the expression of genes related to cell wall and cuticle metabolism during skin-specific ripening program. Our results provide a comprehensive view of the berry skin native polymer composition and modifications during ripening, and allowed identifying candidates genes involved in skin polymer metabolism.

Cell wall dynamic changes of grape berry skin over ripening revealed by ATR-FTIR microspectroscopy

FASOLI, Marianna;DAL SANTO, SILVIA;MONTI, Francesca;ZENONI, Sara
2015

Abstract

FTIR microspectroscopy was used to observe changes in the inner and outer skin sides during the ripening process in cultivar Corvina. Measurements were performed in ATR mode without any sample preparation to preserve the native cell wall structure, with the aim of providing information about the in-muro organization of the cell wall polymers and functional groups. Absorption spectra were analyzed by an automated spectra selection procedure, Principal Component Analysis and graphical heatmap representation of the data, choosing two distinct normalizations for the two skin sides, in order to unravel modifications masked by their distinctive traits. Genome-wide transcriptomic approach was used to profile the expression of genes related to cell wall and cuticle metabolism during skin-specific ripening program. Our results provide a comprehensive view of the berry skin native polymer composition and modifications during ripening, and allowed identifying candidates genes involved in skin polymer metabolism.
Cell wall polymers
Berry skin
FTIR microspectroscopy
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/966451
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