Bioluminescence in vitro studies are usually performed with dedicated microscopes. In this work, we developed a novel image recovery algorithm and a multimodal system prototype to perform bioluminescence microscopy. We performed a feasibility study using GEANT4 Monte Carlo (MC) simulation of bioluminescent cells acquired at low SNR frames and processed using a Super Resolution Regularization Algorithm (SRRA). The method was also tested using in vitro cell acquisition. The results obtained with MC simulations showed an improvement in the spatial resolution from 90 m to 10 m and from 110 m to 13 m for in vitro imaging of mesothelioma cells.

High resolution in vitro bioluminescence imaging using a multimodal optical system

Altabella, L.;BOSCHI, Federico;
2016-01-01

Abstract

Bioluminescence in vitro studies are usually performed with dedicated microscopes. In this work, we developed a novel image recovery algorithm and a multimodal system prototype to perform bioluminescence microscopy. We performed a feasibility study using GEANT4 Monte Carlo (MC) simulation of bioluminescent cells acquired at low SNR frames and processed using a Super Resolution Regularization Algorithm (SRRA). The method was also tested using in vitro cell acquisition. The results obtained with MC simulations showed an improvement in the spatial resolution from 90 m to 10 m and from 110 m to 13 m for in vitro imaging of mesothelioma cells.
2016
Image processing; Multi-modality systems
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/966166
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