In this study, we compare the histological localisation of phenols and b-glucan in six cereal cross sections.The concentrations of b-glucan and total phenols in whole grain ﬂour (WGF) were also investigated toprovide their nutrient content in grams. Cross sections of durum, soft, einkorn and emmer wheat, oatsand barley were stained with Azan Trichrome (ATH) and periodic acid–Schiﬀ (PAS) to characterise themorphology and cytohistology of the living cells in the aleurone, subaleurone and germ, as well as thea-nucleated cells in the starchy endosperm. Phenol localisation in the outer layers was performed by ﬂuo-rescence microscopy due to the presence of ferulic acid. b-Glucan, examined with Calcoﬂuor White stain-ing, was found to be located primarily in the subaleurone layer and starchy endosperm of oats andbarley. Our ﬁndings shed light on why WGF nutritional value is markedly reduced when the bran isremoved through milling.
|Titolo:||The histology of grain caryopses for nutrient location: a comparative study of six cereals|
|Data di pubblicazione:||2017|
|Appare nelle tipologie:||01.01 Articolo in Rivista|
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