Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2-day reduction in average detection time, and 100% specificity.

Extended and direct evaluation of RT-QuIC assays for Creutzfeldt-Jakob disease diagnosis

BONGIANNI, Matilde;FIORINI, Michele;ZANUSSO, Gianluigi;
2017-01-01

Abstract

Real-Time Quaking-Induced Conversion (RT-QuIC) testing of human cerebrospinal fluid (CSF) is highly sensitive and specific in discriminating sporadic CJD patients from those without prion disease. Here, using CSF samples from 113 CJD and 64 non-prion disease patients, we provide the first direct and concurrent comparison of our improved RT-QuIC assay to our previous assay, which is similar to those commonly used internationally for CJD diagnosis. This extended comparison demonstrated a ~21% increase in diagnostic sensitivity, a 2-day reduction in average detection time, and 100% specificity.
2017
Real-Time Quaking-Induced Conversion (RT-QuIC); Creutzfeldt-Jakob disease; diagnostic sensitivity; detection time; specificity
File in questo prodotto:
File Dimensione Formato  
Groveman_et_al-2017-Annals_of_Clinical_and_Translational_Neurology.pdf

accesso aperto

Descrizione: CC BY-NC-ND 4.0 publisher's version
Tipologia: Versione dell'editore
Licenza: Creative commons
Dimensione 162.73 kB
Formato Adobe PDF
162.73 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/958159
Citazioni
  • ???jsp.display-item.citation.pmc??? 33
  • Scopus 74
  • ???jsp.display-item.citation.isi??? 65
social impact