The anti-inflammatory actions of IL-4 are well established through earlier findings. However, the exact mechanism it uses to downregulate the pro-inflammatory cytokine production through monocytes and macrophages is poorly understood. In this study, we examined the effect of IL-4 in the induction of 11β-HSD1 in the two main classes of monocytes, CD14(++) CD16(-) (CD14) and CD14(+) CD16(+) (CD16). Peripheral Blood Mononuclear Cells (PBMCs) were isolated from 17 healthy donors and were sorted into CD14 and CD16 subpopulations using cell sorting. Effect of IL-4 on 11β-HSD1-enzyme activity was measured in sorted and unsorted monocytes using Homogeneous Time-Resolved Fluorescence (HTRF) and M1/M2 polarization analysis was performed by flow cytometry. Our results indicate that CD14 cells are the major source of 11β-HSD1 enzyme after IL-4 stimulation and that M2 phenotype is not a pre-requisite for its synthesis.

CD14(++) CD16(-) monocytes are the main source of 11β-HSD type 1 after IL-4 stimulation

KUNNATHULLY, VIDYA SATHEESN;GOMEZ, Maria Macarena;BASSI, Giulio;POLI, Fabio;ZORATTI, Elisa;LA VERDE, Valentina;IDOLAZZI, Luca;GATTI, Davide;VIAPIANA, Ombretta;ADAMI, Silvano;ROSSINI, Maurizio
2017

Abstract

The anti-inflammatory actions of IL-4 are well established through earlier findings. However, the exact mechanism it uses to downregulate the pro-inflammatory cytokine production through monocytes and macrophages is poorly understood. In this study, we examined the effect of IL-4 in the induction of 11β-HSD1 in the two main classes of monocytes, CD14(++) CD16(-) (CD14) and CD14(+) CD16(+) (CD16). Peripheral Blood Mononuclear Cells (PBMCs) were isolated from 17 healthy donors and were sorted into CD14 and CD16 subpopulations using cell sorting. Effect of IL-4 on 11β-HSD1-enzyme activity was measured in sorted and unsorted monocytes using Homogeneous Time-Resolved Fluorescence (HTRF) and M1/M2 polarization analysis was performed by flow cytometry. Our results indicate that CD14 cells are the major source of 11β-HSD1 enzyme after IL-4 stimulation and that M2 phenotype is not a pre-requisite for its synthesis.
11β-HSD1; anti-inflammatory; glucocorticoids; IL-4; M2; monocytes
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/955799
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