The molecular events that characterize post-ripening grapevine berries have rarely been investigated and are poorly defined. In particular, a detailed definition of changes occurring during the postharvest dehydration, a process undertaken to make some particularly special wine styles, would be of great interest for both winemakers and plant biologists. We report an exhaustive survey of transcriptomic and metabolomic responses in berries representing six grapevine genotypes subjected to postharvest dehydration under identical controlled conditions. The modulation of phenylpropanoid metabolism clearly distinguished the behavior of genotypes with stilbene accumulation as the major metabolic event, although in genotypes that do not accumulate stilbenes the prevalent variation was the transient accumulation/depletion of anthocyanins and flavonols. The modulation of genes related to phenylpropanoid/stilbene metabolism highlighted the distinct metabolomic plasticity of genotypes, allowing the identification of candidate structural and regulatory genes. In addition to genotype-specific responses, a core set of genes was consistently modulated in all genotypes, representing the common features of berries undergoing dehydration and/or commencing senescence. This included genes controlling ethylene and auxin metabolism as well as genes involved in oxidative and osmotic stress, defense responses, anaerobic respiration, cell wall and carbohydrate metabolism. Several transcription factors were identified that may control these shared processes in the postharvest berry. Changes representing both common and genotype-specific responses to postharvest conditions shed light on the cellular processes taking place in harvested berries stored under dehydrating conditions for several months.

Disclosing the molecular basis of the postharvest life of berry in different grapevine genotypes

ZENONI, Sara;FASOLI, Marianna;GUZZO, Flavia;DAL SANTO, SILVIA;Amato, Alessandra;ANESI, Andrea;Commisso, Mauro;CEOLDO, Stefania;AVESANI, Linda;PEZZOTTI, Mario;TORNIELLI, Giovanni Battista
2016-01-01

Abstract

The molecular events that characterize post-ripening grapevine berries have rarely been investigated and are poorly defined. In particular, a detailed definition of changes occurring during the postharvest dehydration, a process undertaken to make some particularly special wine styles, would be of great interest for both winemakers and plant biologists. We report an exhaustive survey of transcriptomic and metabolomic responses in berries representing six grapevine genotypes subjected to postharvest dehydration under identical controlled conditions. The modulation of phenylpropanoid metabolism clearly distinguished the behavior of genotypes with stilbene accumulation as the major metabolic event, although in genotypes that do not accumulate stilbenes the prevalent variation was the transient accumulation/depletion of anthocyanins and flavonols. The modulation of genes related to phenylpropanoid/stilbene metabolism highlighted the distinct metabolomic plasticity of genotypes, allowing the identification of candidate structural and regulatory genes. In addition to genotype-specific responses, a core set of genes was consistently modulated in all genotypes, representing the common features of berries undergoing dehydration and/or commencing senescence. This included genes controlling ethylene and auxin metabolism as well as genes involved in oxidative and osmotic stress, defense responses, anaerobic respiration, cell wall and carbohydrate metabolism. Several transcription factors were identified that may control these shared processes in the postharvest berry. Changes representing both common and genotype-specific responses to postharvest conditions shed light on the cellular processes taking place in harvested berries stored under dehydrating conditions for several months.
2016
Grapevine; postharvest; metabolism; transcriptomics; metabolomics
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/951392
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