Retracting hypertrophic scars resulting from healed burn wounds heavily impact on the patients' life quality. Biomaterial scaffolds guiding burned-out skin regeneration could suppress or lessen scar retraction. Here we report a novel silk noil-based 3D nonwoven scaffold produced by carding-needling with no formic acid exposure which might improve burn healing. Once wetted, it displays human skin-like physical features and a high biocompatibility. Human keratinocyte-like cervical carcinoma C4-I cells seeded onto the carded-needled nonwovens in vitro quickly adhered to them, grew, and actively metabolized glutamine releasing lactate. As on plastic, they released no proinflammatory IL-1β, though secreting TNF-α, an inducer of the autocrine mitogen amphiregulin in such cells. Once grafted into inter-scapular subcutaneous tissue of mice carded-needled nonwovens guided the afresh assembly of a connective tissue enveloping the fibroin microfibers and filling the interposed voids within three months. Fibroblasts and a few poly- or mono-nucleated macrophages populated the engineered tissue. Besides, its extracellular matrix contained thin sparse collagen fibrils and a newly formed vascular network whose endothelin-1-expressing endothelial cells grew first on the fibroin microfibrils and later expanded into the intervening matrix. Remarkably, no infiltrates of inflammatory leukocytes and no packed collagen fibers bundles among fibroin microfibers, no fibrous capsules at the grafts periphery, and hence no foreign body response obtained at the end of three months of observation. Therefore, we posit silk noil-based 3D carded-needled nonwoven scaffolds are tools for Translational Medicine studies as they could guide connective tissue regeneration at deep burn wounds averting scar retraction with good functional results.

BIOCOMPATIBLE SILK NOIL-BASED 3D CARDED-NEEDLED NONWOVEN SCAFFOLDS GUIDE THE ENGINEERING OF NOVEL SKIN CONNECTIVE TISSUE

CHIARINI, Anna Maria;Liu, Daisong;ARMATO, Ubaldo;DAL PRÀ, Ilaria Pierpaola
2016-01-01

Abstract

Retracting hypertrophic scars resulting from healed burn wounds heavily impact on the patients' life quality. Biomaterial scaffolds guiding burned-out skin regeneration could suppress or lessen scar retraction. Here we report a novel silk noil-based 3D nonwoven scaffold produced by carding-needling with no formic acid exposure which might improve burn healing. Once wetted, it displays human skin-like physical features and a high biocompatibility. Human keratinocyte-like cervical carcinoma C4-I cells seeded onto the carded-needled nonwovens in vitro quickly adhered to them, grew, and actively metabolized glutamine releasing lactate. As on plastic, they released no proinflammatory IL-1β, though secreting TNF-α, an inducer of the autocrine mitogen amphiregulin in such cells. Once grafted into inter-scapular subcutaneous tissue of mice carded-needled nonwovens guided the afresh assembly of a connective tissue enveloping the fibroin microfibers and filling the interposed voids within three months. Fibroblasts and a few poly- or mono-nucleated macrophages populated the engineered tissue. Besides, its extracellular matrix contained thin sparse collagen fibrils and a newly formed vascular network whose endothelin-1-expressing endothelial cells grew first on the fibroin microfibrils and later expanded into the intervening matrix. Remarkably, no infiltrates of inflammatory leukocytes and no packed collagen fibers bundles among fibroin microfibers, no fibrous capsules at the grafts periphery, and hence no foreign body response obtained at the end of three months of observation. Therefore, we posit silk noil-based 3D carded-needled nonwoven scaffolds are tools for Translational Medicine studies as they could guide connective tissue regeneration at deep burn wounds averting scar retraction with good functional results.
2016
hypertrophic scar
fibroin
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/947031
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