Continued progress in chronic alcohol abuse investigation requires the development of less invasive procedures for screening purposes. The application of finger-prick and related dried blood spots (fpDBS) for CDT detection appears suitable for this aim. Therefore, the goal of this project was to develop a screening method for CDT using fpDBS with capillary electrophoresis (CZE) analysis.Blood samples prepared by finger-prick were placed on DBS cards and left to air dry; each dried fpDBS disc was shredded into small pieces and suspended in acid solution (60 μL of HCl 120 mmol/L). After centrifugation (10 min at 1500g) the collected sample was adjusted to pH 3.5. After an overnight incubation, the pH was neutralized and an iron rich solution was added. After 1 hour, CZE analysis was carried out. A group of 47 individuals was studied. Parallel serum samples collected from each investigated subject and the %CDT for each sample was measured using high performance liquid chromatography (HPLC) and CZE techniques. The fpDBS transferrin sialo isoform electropherograms were similar to those obtained with serum. Moreover, fpDBS CZE CDT percentage levels demonstrated significant statistical correlation with those obtained from serum for both HPLC and CZE %CDT (p< 0.01; r(2) = 0.8913 and r(2) = 0.8976 respectively), with %CDT from 0.8% to 13.7% for fpDBS and from 0.7% to 12.7% for serum. The newly developed fpDBS procedure for CDT analysis provides a simple and inexpensive tool for use in population screening. This article is protected by copyright. All rights reserved.

The use of finger-prick dried blood spots (fpdbs) and capillary electrophoresis for carbohydrate deficient transferrin (cdt) screening in forensic toxicology

Bertaso A;Sorio D;Vandoros A;De Palo EF;Bortolotti F;Tagliaro F
2016-01-01

Abstract

Continued progress in chronic alcohol abuse investigation requires the development of less invasive procedures for screening purposes. The application of finger-prick and related dried blood spots (fpDBS) for CDT detection appears suitable for this aim. Therefore, the goal of this project was to develop a screening method for CDT using fpDBS with capillary electrophoresis (CZE) analysis.Blood samples prepared by finger-prick were placed on DBS cards and left to air dry; each dried fpDBS disc was shredded into small pieces and suspended in acid solution (60 μL of HCl 120 mmol/L). After centrifugation (10 min at 1500g) the collected sample was adjusted to pH 3.5. After an overnight incubation, the pH was neutralized and an iron rich solution was added. After 1 hour, CZE analysis was carried out. A group of 47 individuals was studied. Parallel serum samples collected from each investigated subject and the %CDT for each sample was measured using high performance liquid chromatography (HPLC) and CZE techniques. The fpDBS transferrin sialo isoform electropherograms were similar to those obtained with serum. Moreover, fpDBS CZE CDT percentage levels demonstrated significant statistical correlation with those obtained from serum for both HPLC and CZE %CDT (p< 0.01; r(2) = 0.8913 and r(2) = 0.8976 respectively), with %CDT from 0.8% to 13.7% for fpDBS and from 0.7% to 12.7% for serum. The newly developed fpDBS procedure for CDT analysis provides a simple and inexpensive tool for use in population screening. This article is protected by copyright. All rights reserved.
2016
Alcohol; CDT; Finger-prick dried blood spot; HPLC; Screening analysis
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/941537
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