AIM:Qtracker®800 Vascular labels (Qtracker®800) are promising biomedical tools for high-resolution vasculature imaging; their effects on mouse and human endothelia, however, are still unknown.MATERIALS & METHODS:Qtracker®800 were injected in Balb/c mice, and brain endothelium uptake was investigated by transmission electron microscopy 3-h post injection. We then investigated, in vitro, the effects of Qtracker®800 exposure on mouse and human endothelial cells by calcium imaging.RESULTS:Transmission electron microscopy images showed nanoparticle accumulation in mouse brain endothelia. A subset of mouse and human endothelial cells generated intracellular calcium transients in response to Qtracker®800.CONCLUSION:Qtracker®800 nanoparticles elicit endothelial functional responses, which prompts biomedical safety evaluations and may bias the interpretation of experimental studies involving vascular imaging.

Are they in or out? The elusive interaction between Qtracker(®)800 vascular labels and brain endothelial cells

Radu, Beatrice Mihaela;Radu, Mihai
;
Tognoli, Cristina;Benati, Donatella;Merigo, Flavia;Assfalg, Michael;Solani, Erika;Stranieri, Chiara;Ceccon, Alberto;Fratta Pasini, Anna Maria;Cominacini, Luciano;Osculati, Francesco;Bertini, Giuseppe;Fabene, Paolo
2015

Abstract

AIM:Qtracker®800 Vascular labels (Qtracker®800) are promising biomedical tools for high-resolution vasculature imaging; their effects on mouse and human endothelia, however, are still unknown.MATERIALS & METHODS:Qtracker®800 were injected in Balb/c mice, and brain endothelium uptake was investigated by transmission electron microscopy 3-h post injection. We then investigated, in vitro, the effects of Qtracker®800 exposure on mouse and human endothelial cells by calcium imaging.RESULTS:Transmission electron microscopy images showed nanoparticle accumulation in mouse brain endothelia. A subset of mouse and human endothelial cells generated intracellular calcium transients in response to Qtracker®800.CONCLUSION:Qtracker®800 nanoparticles elicit endothelial functional responses, which prompts biomedical safety evaluations and may bias the interpretation of experimental studies involving vascular imaging.
calcium imaging, cytosolic calcium transients, human umbilical vein endothelial cells, mouse brain endothelium, nanoparticle uptake, nanoarticles, transmission electron microscopy
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/932005
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