Two analytical approaches for the rapid measurement of hydrogen sulfide (H2S) have been compared to a reference method for their potential application as a rapid procedure for the quantification of H2S formed during alcoholic fermentations. In one case, silver nitrate, lead acetate, and mercuric chloride selective detector tubes for the analysis of H2S in air were investigated. In the other case, a commercially available kit for the diagnosis of nitrogen starvation in wine fermentations, which is based on the detection of H2S, was investigated. Both methods exhibited excellent linearity of response, but the mercuric chloride tube was found to suffer from interferences due to the concomitant presence of mercaptans, which resulted in erroneous H2S quantification. A comparative study between the two methods studied and the cadmium hydroxide/methylene blue reference method commonly used to monitor H2S indicate that the two new methods displayed better recoveries at low H2S concentrations, besides being more rapid and economical. The two new methods were successfully used to quantify production of H2S in different grape juice fermentations. The suitability of each method for the study of specific aspects of H2S production during fermentation is discussed. © 2009.

Comparison of three methods for accurate quantification of hydrogen sulfide during fermentation

Ugliano, Maurizio;
2010-01-01

Abstract

Two analytical approaches for the rapid measurement of hydrogen sulfide (H2S) have been compared to a reference method for their potential application as a rapid procedure for the quantification of H2S formed during alcoholic fermentations. In one case, silver nitrate, lead acetate, and mercuric chloride selective detector tubes for the analysis of H2S in air were investigated. In the other case, a commercially available kit for the diagnosis of nitrogen starvation in wine fermentations, which is based on the detection of H2S, was investigated. Both methods exhibited excellent linearity of response, but the mercuric chloride tube was found to suffer from interferences due to the concomitant presence of mercaptans, which resulted in erroneous H2S quantification. A comparative study between the two methods studied and the cadmium hydroxide/methylene blue reference method commonly used to monitor H2S indicate that the two new methods displayed better recoveries at low H2S concentrations, besides being more rapid and economical. The two new methods were successfully used to quantify production of H2S in different grape juice fermentations. The suitability of each method for the study of specific aspects of H2S production during fermentation is discussed. © 2009.
2010
Alcoholic fermentation; Analytical approach; Comparative studies; Grape juice; Lead acetate; Mercuric chloride; Nitrogen starvation; Potential applications; Rapid measurement; Rapid procedures; Reference method; Selective detectors; Silver nitrates; Wine fermentation; Wine yeast, Cadmium; Chlorine compounds; Detectors; Fermentation; Fruit juices; Hydrogen; Mercury compounds; Metal recovery; Silver; Sulfur determination; Tubes (components); Wine; Yeast, Hydrogen sulfide, cadmium derivative; cadmium hydroxide; hydrogen sulfide; lead acetate; mercuric chloride; methylene blue; silver nitrate; sulfide; sulfur dioxide; thiol derivative; unclassified drug, accuracy; analytic method; analytical error; article; bioaccumulation; chemical analysis; chemical reaction; concentration (parameters); controlled study; fermentation monitoring; intermethod comparison; metabolite; priority journal; product recovery; quantitative analysis, Cadmium; Fermentation; Food Analysis; Hydrogen Sulfide; Mercuric Chloride; Organometallic Compounds; Silver Nitrate; Wine, Vitaceae; Detector tubes; Fermentation; Hydrogen sulfide; Nitrogenius® kit; Wine; Yeast
File in questo prodotto:
File Dimensione Formato  
H2S ACA.pdf

solo utenti autorizzati

Tipologia: Documento in Post-print
Licenza: Accesso ristretto
Dimensione 217.7 kB
Formato Adobe PDF
217.7 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/929333
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 16
social impact