CATALOGO DEI PRODOTTI DELLA RICERCA
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EnglishThe early nodulin N5 of Medicago truncatula is a lipid transfer protein that has been proven to positively regulate nodulation, although it displays in vitro inhibitory activity against Sinorhizobium meliloti. We utilized a RNAi-based hairpin construct to down-regulate MtN5 expression in M. truncatula hairy roots. MtN5-silenced roots inoculated with rhizobia displayed an increased number of root hair curling events and a reduced number of invaded primordia and mature nodules as compared to wild type roots. Nodule primordia formation appeared unaltered in MtN5-silenced roots. This phenotype was associated with the stimulation of ENOD11 expression, an early marker of infection, and with the down-regulation of Flotillin 4(FLOT4), a protein involved in rhizobia entry pathway.
| Titolo: | Involvement of Medicago truncatula lipid transfer protein N5 in epidermal phases of rhizobia-host interaction. |
| Autori: | |
| Data di pubblicazione: | 2012 |
| Abstract: | The early nodulin N5 of Medicago truncatula is a lipid transfer protein that has been proven to positively regulate nodulation, although it displays in vitro inhibitory activity against Sinorhizobium meliloti. We utilized a RNAi-based hairpin construct to down-regulate MtN5 expression in M. truncatula hairy roots. MtN5-silenced roots inoculated with rhizobia displayed an increased number of root hair curling events and a reduced number of invaded primordia and mature nodules as compared to wild type roots. Nodule primordia formation appeared unaltered in MtN5-silenced roots. This phenotype was associated with the stimulation of ENOD11 expression, an early marker of infection, and with the down-regulation of Flotillin 4(FLOT4), a protein involved in rhizobia entry pathway. |
| Handle: | http://hdl.handle.net/11562/876782 |
| Appare nelle tipologie: | 04.02 Abstract in Atti di convegno |
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