CDT is a widely recognized marker of chronic abuse of alcohol, ued in both clinical and forensic settings. CDT identifies a group of minor glycoforms of transferrin, the major iron transporting serum protein. Sound research has indicated that subjects with an alcohol intake > 60-80 grams per days for at least 7 consecutive days show increased CDT concentrazion. At present, the analytical methods for CDT determination include immunoassays and methods based on capillary electrophoresis and HPLC. In order to achieved unequivocal recognition of CDT peaks, an automated and reproducible in-capillary immunosubtraction was developed by injectin a plug of serum sample followed by a plug of anti-Tf antiserum.
Innovation in the analysis of carbohydrate deficient transferrin (CDT) with capillary electrophoresis
SORIO, DANIELA;DE PALO, Elio Franco;BORTOLOTTI, Federica;TAGLIARO, Franco
2012-01-01
Abstract
CDT is a widely recognized marker of chronic abuse of alcohol, ued in both clinical and forensic settings. CDT identifies a group of minor glycoforms of transferrin, the major iron transporting serum protein. Sound research has indicated that subjects with an alcohol intake > 60-80 grams per days for at least 7 consecutive days show increased CDT concentrazion. At present, the analytical methods for CDT determination include immunoassays and methods based on capillary electrophoresis and HPLC. In order to achieved unequivocal recognition of CDT peaks, an automated and reproducible in-capillary immunosubtraction was developed by injectin a plug of serum sample followed by a plug of anti-Tf antiserum.
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