In this study, a rapid chiral separation of 12 cathinones analogs has been developed and validated using cyclodextrin-assisted CE with UV and TOF-MS detection. Optimum separation was obtained on a 57.5 cm × 50 m capillary using a buffer system consisting of 10 mM -cyclodextrin (-CD) in a 100 mM phosphate buffer for CE-UV, and 0.6% v/v highlysulfated--cyclodextrin (HS--CD) in a 50 mM phosphate buffer for CE-MS. In the CE-MS experiment, a partial filling technique was employed to ensure that a minimum amount of cyclodextrin entered the mass spectrometer. All analytes were separated within 18 min inthe CE-UV separation and identified by TOF-MS. Ten compounds were enantiomerically separated using -CD in the UV mode and an additional two more were enantiomerically separated using HS--CD in the MS mode. Detection limits down to 1.0 ng/mL were obtained. The method was then applied to examine seized drugs.

Chiral separation of 12 cathinone analogs by cyclodextrin-assisted capillary electrophoresis with UV and mass spectrometry detection

TAGLIARO, Franco;
2014

Abstract

In this study, a rapid chiral separation of 12 cathinones analogs has been developed and validated using cyclodextrin-assisted CE with UV and TOF-MS detection. Optimum separation was obtained on a 57.5 cm × 50 m capillary using a buffer system consisting of 10 mM -cyclodextrin (-CD) in a 100 mM phosphate buffer for CE-UV, and 0.6% v/v highlysulfated--cyclodextrin (HS--CD) in a 50 mM phosphate buffer for CE-MS. In the CE-MS experiment, a partial filling technique was employed to ensure that a minimum amount of cyclodextrin entered the mass spectrometer. All analytes were separated within 18 min inthe CE-UV separation and identified by TOF-MS. Ten compounds were enantiomerically separated using -CD in the UV mode and an additional two more were enantiomerically separated using HS--CD in the MS mode. Detection limits down to 1.0 ng/mL were obtained. The method was then applied to examine seized drugs.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11562/871583
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