Total body fat and central fat mass independently predict insulin resistance but not hyperandrogenemia in women with polycystic ovary syndrome.

Context/Objective: Obesity is a common feature of women with polycystic ovary syndrome (PCOS). The aim of this study was to assess the role of body fat on insulin resistance and androgen excess in these subjects. Patients/Design: One hundred sixteen consecutive Caucasian women with PCOS, diagnosed by the Rotterdam criteria, underwent accurate assessment of clinical, anthropometric, hormonal and metabolic features. In particular, total fat mass and fat distribution were assessed by dual-energy x-ray absorptiometry, serum free testosterone by liquid chromatography mass spectrometry and equilibrium dialysis, and insulin sensitivity by the glucose clamp technique.Results: Total fat mass and truncal fat were significantly higher in insulin-resistant than in insulin-sensitive PCOS subjects (+89% and +127%, respectively, both p<0.001) and both tended to be higher in hyperandrogenemic than in normoandrogenemic women (+22% and +28%, respectively, p=0.087 and p=0.090). All parameters of adiposity correlated inversely with insulin sensitivity (p<0.001) and directly with serum free testosterone (p≤0.001). A statistically significant inverse relationship was observed between insulin sensitivity and serum free testosterone concentrations (r=-0.527, p<0.001).In multiple regression analysis, either total fat mass or truncal fat, in addition to serum free testosterone and age, were independent predictors of insulin sensitivity. However, insulin sensitivity, but not total fat mass or truncal fat, was an independent predictor of free testosterone concentrations.Conclusions: These data suggest that body fat contributes to determining insulin resistance in PCOS women. However, the association between body fat and hyperandrogenism seems to a large extent explained by insulin resistance.