Mammalian cleavage factor I (CF Im) is an essential factor that is required for the first step in pre-mRNA 3 end processing.Here, we characterize CF Im68 subnuclear distribution and mobility. Fluorescence microscopy reveals that in addition toparaspeckles CF Im68 accumulates in structures that partially overlap with nuclear speckles. Analysis of synchronizedcells shows that CF Im68 distribution in speckles and paraspeckles varies during the cell cycle. At an ultrastructural level,CF Im68 is associated with perichromatin fibrils, the sites of active transcription, and concentrates in interchromatingranules-associated zones. We show that CFIm68 colocalizes with bromouridine, RNA polymerase II, and the splicingfactor SC35. On inhibition of transcription, endogenous CF Im68 no longer associates with perichromatin fibrils, but it canstill be detected in interchromatin granules-associated zones. These observations support the idea that not only splicingbut also 3 end processing occurs cotranscriptionally. Finally, fluorescence recovery after photobleaching analysis revealsthat the CF Im68 fraction associated with paraspeckles moves at a rate similar to the more dispersed molecules in thenucleoplasm, demonstrating the dynamic nature of this compartment. These findings suggest that paraspeckles are afunctional compartment involved in RNA metabolism in the cell nucleus.
|Titolo:||Subnuclear localization and dynamics of the Pre-mRNA 3' end processing factor mammalian cleavage factor I 68-kDa subunit.|
|Data di pubblicazione:||2007|
|Appare nelle tipologie:||01.01 Articolo in Rivista|