INTRODUCTION AND AIM OF THE STUDY The Peyronie's disease (PD) is an idiopathic disorder of connective tissue ot the penis, that involves the tunica albuginea of the corpora cavernosa and the adjacent areolar space. Many studies have tried to identify risk factors associated with PD and the role of inflammation and free radicals in the pathogenesis of plaque seems to be important. It is a growing clinical evidence to support the therapeutic potential of mesenchymal stem cells for the revascularization of ischemic tissues and the recovery of their function and histological findings has assumed a possible application of lipofilling technique in patients with PD. The objective of this experimental study is the creation of a murine experimental model of PD, evaluating with MRI the penis of the rats (feasibility study), in order to plane the application of lipofilling technique in an animal model. MATERIALS and METHODS Four male Wistar rats were anesthetized, fixed in prone position and subjected to MRI; image acquisition was performed using a scanner Biospec (Bruker, Karlsruhe, Germany) equipped with a horizontal magnet operating at 4.7 Tesla with opening of 33 cm (Oxford Ltd, Oxford, UK). The animals underwent, subsequently, an injection of thrombin in the tunica albuginea, using a 1 mL syringe with needle 30 G, opening the dartos. MRI images were acquired in the same manner as described above at 7 and 21 days after injection with incision of the Dartos RESULTS The MRI acquisitions, both in coronal and axial projection, showed an adequate visibility of the anatomical structures. At 7 days after thrombin injection with the Dartos incision it was evident an oedematous portion, visible as a hyperintense area, located at the injection area. At 21 days after injection, oedema was partially resolved: the injection part of the hyperintense area remains unchanged, while the remaining area appears to be part of a re-absorption and re-organization process. Since none of the various treatment modalities currently available for the management of PD is able to bring healing, the researchers' attention is increasingly directed towards innovative treatment programs, such as the use of stem cells of mesenchymal origin. Since there is no data in the literature concerning the role of stem cells in management of PD, we planned to develop a murine model of stable IPP, monitored with imaging (MRI). At the present time, the research in PD is hampered by the lack of universally accepted animal model and this is likely attributed to the limited insight into PD mechanisms and the difficulties faced by current animal models to truly represent the complexity and complete spectrum of human disease.
MURINE EXPERIMENTAL MODEL OF PEYRONIE'S DISEASE: A PILOT STUDY
D'ELIA, Carolina;CERRUTO, Maria Angela;Cavicchioli, Francesca Maria;ARTIBANI, Walter
2013-01-01
Abstract
INTRODUCTION AND AIM OF THE STUDY The Peyronie's disease (PD) is an idiopathic disorder of connective tissue ot the penis, that involves the tunica albuginea of the corpora cavernosa and the adjacent areolar space. Many studies have tried to identify risk factors associated with PD and the role of inflammation and free radicals in the pathogenesis of plaque seems to be important. It is a growing clinical evidence to support the therapeutic potential of mesenchymal stem cells for the revascularization of ischemic tissues and the recovery of their function and histological findings has assumed a possible application of lipofilling technique in patients with PD. The objective of this experimental study is the creation of a murine experimental model of PD, evaluating with MRI the penis of the rats (feasibility study), in order to plane the application of lipofilling technique in an animal model. MATERIALS and METHODS Four male Wistar rats were anesthetized, fixed in prone position and subjected to MRI; image acquisition was performed using a scanner Biospec (Bruker, Karlsruhe, Germany) equipped with a horizontal magnet operating at 4.7 Tesla with opening of 33 cm (Oxford Ltd, Oxford, UK). The animals underwent, subsequently, an injection of thrombin in the tunica albuginea, using a 1 mL syringe with needle 30 G, opening the dartos. MRI images were acquired in the same manner as described above at 7 and 21 days after injection with incision of the Dartos RESULTS The MRI acquisitions, both in coronal and axial projection, showed an adequate visibility of the anatomical structures. At 7 days after thrombin injection with the Dartos incision it was evident an oedematous portion, visible as a hyperintense area, located at the injection area. At 21 days after injection, oedema was partially resolved: the injection part of the hyperintense area remains unchanged, while the remaining area appears to be part of a re-absorption and re-organization process. Since none of the various treatment modalities currently available for the management of PD is able to bring healing, the researchers' attention is increasingly directed towards innovative treatment programs, such as the use of stem cells of mesenchymal origin. Since there is no data in the literature concerning the role of stem cells in management of PD, we planned to develop a murine model of stable IPP, monitored with imaging (MRI). At the present time, the research in PD is hampered by the lack of universally accepted animal model and this is likely attributed to the limited insight into PD mechanisms and the difficulties faced by current animal models to truly represent the complexity and complete spectrum of human disease.
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