The aim of this work is to investigate the role of N-acetylornithine deacetylase (NAOD) in fruit development. To study its biological role, we have generated silenced lines by transforming Arabidopsis plants with a hairpin construct and searched for T-DNA null mutants. We obtained two silenced lines that showed approximately 90%reduction in NAOD transcript level in comparison with wild type. The phenotypical analysis of these lines revealed a reduced growth and a lower number of siliques in comparison with control plants. A T-DNA insertional line was obtained from the SALK collection. The expression of the gene was almost completely abolished in the homozygous mutant line.
Titolo: | Study of the role of a putative acetylornithine deacetylase on fruit development in Arabidopsis thaliana |
Autori: | |
Data di pubblicazione: | 2013 |
Abstract: | The aim of this work is to investigate the role of N-acetylornithine deacetylase (NAOD) in fruit development. To study its biological role, we have generated silenced lines by transforming Arabidopsis plants with a hairpin construct and searched for T-DNA null mutants. We obtained two silenced lines that showed approximately 90%reduction in NAOD transcript level in comparison with wild type. The phenotypical analysis of these lines revealed a reduced growth and a lower number of siliques in comparison with control plants. A T-DNA insertional line was obtained from the SALK collection. The expression of the gene was almost completely abolished in the homozygous mutant line. |
Handle: | http://hdl.handle.net/11562/761361 |
Appare nelle tipologie: | 04.02 Abstract in Atti di convegno |