Mitogen-activated protein kinases (MAPKs) are in downstream phosphorylation/ dephosphorylation cascade of the three-level MAPKKK-MAPKK-MAPK chain, and have been found to play essential roles in mediating biotic and abiotic stress responses in plants. In this study, we identified and annotated all 12 MAPK genes from the 12X V1 sequenced grapevine genome (nominated as VvMAPK) and validated them by cloning their open reading frame (ORF) of cDNA sequences through polymerase chain reaction (PCR) amplification. The VvMAPK family was analyzed bioinformatically in terms of their chromosome locations, sequence homologies, gene structures and phylogeny. As with 20 MAPKs from Arabidopsis, the12 VvMAPK genes are classified into four subfamilies and genes within the same subfamily have similar exon/intron structures. All VvMAPK proteins in subfamily A, B and C have a Thr-Glu-Tyr (TEY) activation domain, whereas those in the group D contain a Thr-Asp-Tyr (TDY) activation domain. Expression analyses of VvMAPK genes using microarray analysis and quantitative real-time PCR demonstrated that all VvMAPK genes are expressed during grapevine growth and development. Based on expression analyses of grapevine tissues and organs at different developmental stages, and of leaf tissues treated with Erysiphenecator (powdery mildew), salicylic acid, ethylene, hydrogen peroxide and drought, we identified several VvMAPK candidate genes which might be involved in grapevine growth and development and in biotic and abiotic responses for further functional characterization.

Genome-wide manual annotation, verification by isolation and expression analyses of MAPK gene family in grapevine (Vitis vinifera)

LOVATO, Arianna;TORNIELLI, Giovanni Battista;POLVERARI, Annalisa;PEZZOTTI, Mario;
In corso di stampa

Abstract

Mitogen-activated protein kinases (MAPKs) are in downstream phosphorylation/ dephosphorylation cascade of the three-level MAPKKK-MAPKK-MAPK chain, and have been found to play essential roles in mediating biotic and abiotic stress responses in plants. In this study, we identified and annotated all 12 MAPK genes from the 12X V1 sequenced grapevine genome (nominated as VvMAPK) and validated them by cloning their open reading frame (ORF) of cDNA sequences through polymerase chain reaction (PCR) amplification. The VvMAPK family was analyzed bioinformatically in terms of their chromosome locations, sequence homologies, gene structures and phylogeny. As with 20 MAPKs from Arabidopsis, the12 VvMAPK genes are classified into four subfamilies and genes within the same subfamily have similar exon/intron structures. All VvMAPK proteins in subfamily A, B and C have a Thr-Glu-Tyr (TEY) activation domain, whereas those in the group D contain a Thr-Asp-Tyr (TDY) activation domain. Expression analyses of VvMAPK genes using microarray analysis and quantitative real-time PCR demonstrated that all VvMAPK genes are expressed during grapevine growth and development. Based on expression analyses of grapevine tissues and organs at different developmental stages, and of leaf tissues treated with Erysiphenecator (powdery mildew), salicylic acid, ethylene, hydrogen peroxide and drought, we identified several VvMAPK candidate genes which might be involved in grapevine growth and development and in biotic and abiotic responses for further functional characterization.
Grapevine; Mitogen-activated protein kinase (MAPK); Gene expression; Powdery mildew; Environmental stress
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11562/741797
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