Urine particles examination: comparison of automated flow citometer UF-1000i and manual quantitative microscopy

Manoni, F.; Ferrian, Melissa; Gusella, G.; Ercolin, M.; Tinello, A.; Fornasiero, L.; Valverde, S.; Gessoni, G.

Aim. Some automated instruments examining urine sediment have been introduced. We compared the performance of Sysmex UF-1000i with manual microscopy in urine particles examination. Methods. 15 urine samples were selected. Urine sediments were examined by manual microscopy by using Fuchs-Rosenthal chamber and UF-1000i analyzer. Results. The within-run CVs for urine samples ranged from 0.8% to 23.1% for the UF-1000i. The agreement between methods was good for red blood cells and white blood cells counts based on r values of 0.99, for epithelial cell the r value was 0.98 and 0.87 for casts. Conclusion. This automated urinalysis system demonstrated good concordance with manual quantitative microscopy. Thus, when combined with urine chemistry analysis, this analyzer might provide a rapid and accurate screening tool in routine urine analysis.

Titolo:	Urine particles examination: comparison of automated flow citometer UF-1000i and manual quantitative microscopy
Autori:	Manoni F. Ferrian, Melissa Gusella G. Ercolin M. Tinello A. Fornasiero L. Valverde S. Gessoni G. mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2009
Rivista:	MINERVA MEDICA
Abstract:	Aim. Some automated instruments examining urine sediment have been introduced. We compared the performance of Sysmex UF-1000i with manual microscopy in urine particles examination. Methods. 15 urine samples were selected. Urine sediments were examined by manual microscopy by using Fuchs-Rosenthal chamber and UF-1000i analyzer. Results. The within-run CVs for urine samples ranged from 0.8% to 23.1% for the UF-1000i. The agreement between methods was good for red blood cells and white blood cells counts based on r values of 0.99, for epithelial cell the r value was 0.98 and 0.87 for casts. Conclusion. This automated urinalysis system demonstrated good concordance with manual quantitative microscopy. Thus, when combined with urine chemistry analysis, this analyzer might provide a rapid and accurate screening tool in routine urine analysis.
Handle:	http://hdl.handle.net/11562/676761
Appare nelle tipologie:	01.01 Articolo in Rivista

File in questo prodotto:

Non ci sono file associati a questo prodotto.

Utilizza questo identificativo per citare o creare un link a questo documento:
http://hdl.handle.net/11562/676761

Citazioni

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

CATALOGO DEI PRODOTTI DELLA RICERCA

File in questo prodotto: