Aim. Some automated instruments examining urine sediment have been introduced. We compared the performance of Sysmex UF-1000i with manual microscopy in urine particles examination. Methods. 15 urine samples were selected. Urine sediments were examined by manual microscopy by using Fuchs-Rosenthal chamber and UF-1000i analyzer. Results. The within-run CVs for urine samples ranged from 0.8% to 23.1% for the UF-1000i. The agreement between methods was good for red blood cells and white blood cells counts based on r values of 0.99, for epithelial cell the r value was 0.98 and 0.87 for casts. Conclusion. This automated urinalysis system demonstrated good concordance with manual quantitative microscopy. Thus, when combined with urine chemistry analysis, this analyzer might provide a rapid and accurate screening tool in routine urine analysis.