The start-up of the completely autotrophic nitrogen removal process was examined in a sequencing batch reactor (SBR) using low activity anoxic ammonium oxidation (anammox) inoculum. The SBR received effluent from an upflow anaerobic sludge blanket (UASB) that treated low strength wastewater. The volumetric nitrogen loading rate (vNLR) was first 0.24±0.11kg Nm-3d-1 and then reduced to 0.10±0.02 kg Nm-3d-1. The average specific anammox activity was 2.27±1.31mg N (gVSS h)-1, at 30°C representing an increase of 161% compared to the inoculum. The decrease in vNLR did not significantly affect anammox activity, but resulted in a decrease of denitrifying heterotrophic activity to very low levels after the first 30days owing to the decrease of organic loading rate (OLR). Fluorescence in situ hybridization (FISH) analysis confirmed the stable presence of anammox bacteria in biomass. Numerous filamentous microorganisms were present, several of which were in a state of endogenous respiration.

Start-up of the completely autotrophic nitrogen removal process using low activity anammox inoculum to treat low strength UASB effluent

Malamis, Symeon Alexandros;Katsou, Evangelia;FRISON, Nicola;DI FABIO, Silvia;FATONE, Francesco
2013

Abstract

The start-up of the completely autotrophic nitrogen removal process was examined in a sequencing batch reactor (SBR) using low activity anoxic ammonium oxidation (anammox) inoculum. The SBR received effluent from an upflow anaerobic sludge blanket (UASB) that treated low strength wastewater. The volumetric nitrogen loading rate (vNLR) was first 0.24±0.11kg Nm-3d-1 and then reduced to 0.10±0.02 kg Nm-3d-1. The average specific anammox activity was 2.27±1.31mg N (gVSS h)-1, at 30°C representing an increase of 161% compared to the inoculum. The decrease in vNLR did not significantly affect anammox activity, but resulted in a decrease of denitrifying heterotrophic activity to very low levels after the first 30days owing to the decrease of organic loading rate (OLR). Fluorescence in situ hybridization (FISH) analysis confirmed the stable presence of anammox bacteria in biomass. Numerous filamentous microorganisms were present, several of which were in a state of endogenous respiration.
Anammox; Autotrophic nitrogen removal; Low strength UASB effluent; Nitritation; Sequencing batch reactor
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11562/620956
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 18
  • ???jsp.display-item.citation.isi??? ND
social impact