Burkholderia fungorum DBT1, first isolated from settling particulate matter of an oil refinery wastewater, is a bacterial strain which has been shown capable of utilizing several polycyclic aromatic hydrocarbons (PAHs) including dibenzothiophene (DBT). In particular, this microbe is able to efficiently degrade DBT through the Kodama pathway. Previous investigations have lead to the identification of six genes, on a total of eight, required for DBT degradation. In the present study, a combined experimental/computational approach was adopted to identify and in silico characterize the two missing genes, namely a ferredoxin reductase and a hydratase-aldolase. Thus, the finding of all enzymatic components of the Kodama pathway in B. fungorum DBT1 makes this bacterial strain amenable for possible exploitation in soil bioremediation protocols

Identification of aldolase and ferredoxin reductase within the dbt operon of Burkholderia fungorum DBT1

Piccoli, Stefano;ANDREOLLI, Marco;GIORGETTI, ALEJANDRO;ZORDAN, Fabio;LAMPIS, Silvia;VALLINI, Giovanni
2014

Abstract

Burkholderia fungorum DBT1, first isolated from settling particulate matter of an oil refinery wastewater, is a bacterial strain which has been shown capable of utilizing several polycyclic aromatic hydrocarbons (PAHs) including dibenzothiophene (DBT). In particular, this microbe is able to efficiently degrade DBT through the Kodama pathway. Previous investigations have lead to the identification of six genes, on a total of eight, required for DBT degradation. In the present study, a combined experimental/computational approach was adopted to identify and in silico characterize the two missing genes, namely a ferredoxin reductase and a hydratase-aldolase. Thus, the finding of all enzymatic components of the Kodama pathway in B. fungorum DBT1 makes this bacterial strain amenable for possible exploitation in soil bioremediation protocols
Burkholderia fungorum DBT1; DIBENZOTHIOPHENE; POLYCYCLIC AROMATIC HYDROCARBONS; TARGETING GENE WALKING; PROTEIN STRUCTURE BIOINFORMATICS
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11562/605558
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