The aim of this study was to evaluate the performance of Sysmex XE-2100 for automated flow cytometric analysis of biological fluids. The results of 106 consecutive peritoneal samples and 20 pleural samples were compared with optical microscopy. A good agreement was found in total nucleated cells (TNC) count between Sysmex XE-2100 and manual microscopy (y=0.98x+3.2, r=0.99; mean bias, -9.5 TNC/μL). The percentage of cells with high luorescence in XE-2100 correlated with that of macrophages and mesothelial cells at microscopy (r=0.67; P <0.001). The CV of TNC determination on XE-2100 ranged between 1.6% and 11.7%. At the 20% CV, the analytical sensitivity of XE-2100 was 29/μL for TNC, 50/μL for polymorphonuclear leukocytes (PMN) and 66/μL for mononuclear elements. The linearity between 30 and 944 TNC/μL was excellent (r=0.998, P <0.001), whereas the carry-over was <1%. At thresholds of ≥250 PMN/μL in peritoneal fluid and ≥50% PMN or ≥50% lymphocytes in pleural fluid, the diagnostic accuracy of XE-2100 was 96.8%, with Cohen's kappa of 0.90 (P <0.001). We conclude that automated flow cytometric analysis on Sysmex XE-2100 may be a viable options for screening of peritoneal and pleural fluids.

Evaluation of Sysmex XE-2100 for enumeration and differentiation of cellular elements in peritoneal and pleural fluids.

LIPPI, Giuseppe
2013

Abstract

The aim of this study was to evaluate the performance of Sysmex XE-2100 for automated flow cytometric analysis of biological fluids. The results of 106 consecutive peritoneal samples and 20 pleural samples were compared with optical microscopy. A good agreement was found in total nucleated cells (TNC) count between Sysmex XE-2100 and manual microscopy (y=0.98x+3.2, r=0.99; mean bias, -9.5 TNC/μL). The percentage of cells with high luorescence in XE-2100 correlated with that of macrophages and mesothelial cells at microscopy (r=0.67; P <0.001). The CV of TNC determination on XE-2100 ranged between 1.6% and 11.7%. At the 20% CV, the analytical sensitivity of XE-2100 was 29/μL for TNC, 50/μL for polymorphonuclear leukocytes (PMN) and 66/μL for mononuclear elements. The linearity between 30 and 944 TNC/μL was excellent (r=0.998, P <0.001), whereas the carry-over was <1%. At thresholds of ≥250 PMN/μL in peritoneal fluid and ≥50% PMN or ≥50% lymphocytes in pleural fluid, the diagnostic accuracy of XE-2100 was 96.8%, with Cohen's kappa of 0.90 (P <0.001). We conclude that automated flow cytometric analysis on Sysmex XE-2100 may be a viable options for screening of peritoneal and pleural fluids.
pleural fluids; screening; ascites
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11562/601351
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