Human CCAAT displacement protein (CDP), a putative repressor of developmentally regulated gene expression, was purified fron HeLa cells by DNA binding-site affinity chromatography. cDNA encoding CDP was obtained by immunoscreening a lambda gt11 library with antibody raised against purified protein. The deduced primary amino acid sequence of CDP reveals remarkable homology to Drosophila cut with respect to the presence of a unique homeodomain and "cut repeats". As cut participates in determination of cell fate in several tissues in Drosophila, the similarity predicts a broad role for CDP in mammalian development.

Human CCAAT displacement protein is homologous to the Drosophila homeoprotein, cut.

LIEVENS, Patricia;
1992

Abstract

Human CCAAT displacement protein (CDP), a putative repressor of developmentally regulated gene expression, was purified fron HeLa cells by DNA binding-site affinity chromatography. cDNA encoding CDP was obtained by immunoscreening a lambda gt11 library with antibody raised against purified protein. The deduced primary amino acid sequence of CDP reveals remarkable homology to Drosophila cut with respect to the presence of a unique homeodomain and "cut repeats". As cut participates in determination of cell fate in several tissues in Drosophila, the similarity predicts a broad role for CDP in mammalian development.
CDP transcription factor; gene cloning
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11562/581558
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