c-fos is a transcriptional regulatory factor that can be rapidly and transiently induced in brain cells in response to a variety of stimuli. However, we observed that c-fos mRNA, in absence of exogenous stimulation, oscillates in different regions of the rat brain in relation to the rest-activity and sleep-wake circadian cycle (Grassi-Zucconi et al, 1993).This previous study was based on Northern blot analysis and did not provide any information on the neuronal cell populations subserving the circadian c-fos oscillation. Therefore, we used immuno- cytochemistry to detect in the rat brain the cells that express the Fos nuclear protein, encoded by the c-fos gene, during spontaneous wakefulness and sleep.The present report derives from analysis of the cerebral cortex; the findings observed at subcortical levels will be described elsewhere.
c-fos spontaneous expression during wakefulness is reversed during sleep in neuronal subsets of the rat cortex
Bentivoglio M.
1994-01-01
Abstract
c-fos is a transcriptional regulatory factor that can be rapidly and transiently induced in brain cells in response to a variety of stimuli. However, we observed that c-fos mRNA, in absence of exogenous stimulation, oscillates in different regions of the rat brain in relation to the rest-activity and sleep-wake circadian cycle (Grassi-Zucconi et al, 1993).This previous study was based on Northern blot analysis and did not provide any information on the neuronal cell populations subserving the circadian c-fos oscillation. Therefore, we used immuno- cytochemistry to detect in the rat brain the cells that express the Fos nuclear protein, encoded by the c-fos gene, during spontaneous wakefulness and sleep.The present report derives from analysis of the cerebral cortex; the findings observed at subcortical levels will be described elsewhere.
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