STR profiling of animal species has a wide range of applications, including forensic identification, wildlife preservation, veterinary public health protection and food safety.We tested the efficacy of a multiplex PCRbased assay including 11 porcine-specific tetrameric STRs in a population sample of wild boars (n = 142) originating from Piedmont (North West Italy). Multiple deviations from Hardy–Weinberg expectations were observed, mostly due to a reduction in observed heterozygosity indicative of a high degree of inbreeding.A value of u of 0.046 and an inbreeding coefficient of 0.089 were estimated.Combined power of discrimination and probability of exclusion values for the STR panel were 0.9999999999996 and 0.99989. In order to test the suitability of themethod formeat traceability purposes, adomestic pig referencesample (n = 412), consisting of commercial lines commonly used in the meat production process, was also typed. A Bayesian cluster analysis carried out using the observed genotypes, showed a percentage of correct subspecies assignment of individual samples of 0.974 for wild boars and 0.991 for pigs, thusdemonstrating the usefulness of the multiplex STR-typing system for discrimination purposes.

Analysis of 11 tetrameric STRs in wild boars for forensic purposes.

Caratti, Stefano;
2010

Abstract

STR profiling of animal species has a wide range of applications, including forensic identification, wildlife preservation, veterinary public health protection and food safety.We tested the efficacy of a multiplex PCRbased assay including 11 porcine-specific tetrameric STRs in a population sample of wild boars (n = 142) originating from Piedmont (North West Italy). Multiple deviations from Hardy–Weinberg expectations were observed, mostly due to a reduction in observed heterozygosity indicative of a high degree of inbreeding.A value of u of 0.046 and an inbreeding coefficient of 0.089 were estimated.Combined power of discrimination and probability of exclusion values for the STR panel were 0.9999999999996 and 0.99989. In order to test the suitability of themethod formeat traceability purposes, adomestic pig referencesample (n = 412), consisting of commercial lines commonly used in the meat production process, was also typed. A Bayesian cluster analysis carried out using the observed genotypes, showed a percentage of correct subspecies assignment of individual samples of 0.974 for wild boars and 0.991 for pigs, thusdemonstrating the usefulness of the multiplex STR-typing system for discrimination purposes.
Sus scrofa; Short tandem repeat (STR); Forensic Genetics
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11562/509150
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