A short gas-chromatographic method for analysing urinary concentrations of 2,5-hexanedione is based on acid hydrolysis of urine at pH below 0.1 and "purification" of the urine samples by microcolumns containing an octadecyl-silane phase. A 5% acetonitrile solution allows a fairly selective elution of 2,5-hexanedione from the microcolumns. Recovery of 2,5-hexanedione from urine is as great as 79.9%. The variation coefficient of the measurements is 2.8%. The results obtained from different working conditions and using packed or wide bore or capillary gas-chromatographic columns are reported.
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