Mantle cell lymphoma shows a peculiar immunogenetic profile, but the functional consequences of this fact are unknown. We have determined the precise sequences of rearranged heavy and light chain genes in several mantle cell lymphoma cell lines and investigated the presence of heavy and light chain stereotypy. These cell lines use IGHV and IGLV genes that are known to be preferentially rearranged in mantle cell lymphoma, but we found no evidence of heavy chain stereotypy. On the contrary, one cell line (Mino) showed a nearly identical light chain complementarity determining region 3 when compared to the only published light chain cluster. Two cell lines couples (Jeko-1/UPN-2 and JVM-2/JVM-13) showed a highly similar light chain, which satisfied the criteria for stereotypy. Our data show that mantle cell lymphoma cell lines resemble the IGHV and IGLV usage of mantle cell lymphoma, and foster the hypothesis that light chain stereotypy might be under-recognized.

Mantle cell lymphoma cell lines show no evident immunoglobulin heavy chain stereotypy but frequent light chain stereotypy

PIGHI, Chiara;BARBI, Stefano;BERTOLASO, Anna;ZAMO', Alberto
2013

Abstract

Mantle cell lymphoma shows a peculiar immunogenetic profile, but the functional consequences of this fact are unknown. We have determined the precise sequences of rearranged heavy and light chain genes in several mantle cell lymphoma cell lines and investigated the presence of heavy and light chain stereotypy. These cell lines use IGHV and IGLV genes that are known to be preferentially rearranged in mantle cell lymphoma, but we found no evidence of heavy chain stereotypy. On the contrary, one cell line (Mino) showed a nearly identical light chain complementarity determining region 3 when compared to the only published light chain cluster. Two cell lines couples (Jeko-1/UPN-2 and JVM-2/JVM-13) showed a highly similar light chain, which satisfied the criteria for stereotypy. Our data show that mantle cell lymphoma cell lines resemble the IGHV and IGLV usage of mantle cell lymphoma, and foster the hypothesis that light chain stereotypy might be under-recognized.
mantle cell lymphoma; B-cell receptor; immunogenetics; stereotypy
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11562/491152
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