Double Domain Swapping in Bovine Seminal RNase: Formation of Distinct N- and C-swapped Tetramers and Multimers with Increasing Biological Activities

Bovine seminal (BS) RNase, the unique natively dimeric member of the RNase super-family, represents a special case notonly for its additional biological actions but also for the singular features of 3D domain swapping. The native enzyme isindeed a mixture of two isoforms: M= M, a dimer held together by two inter-subunit disulfide bonds, and MxM, 70% ofthe total, which, besides the two mentioned disulfides, is additionally stabilized by the swapping of its N-termini. Whenlyophilized from 40% acetic acid, BS-RNase oligomerizes as the super-family proto-type RNase A does. In this paper, weinduced BS-RNase self-association and analyzed the multimers by size-exclusion chromatography, cross-linking,electrophoresis, mutagenesis, dynamic light scattering, molecular modelling. Finally, we evaluated their enzymatic andcytotoxic activities. Several BS-RNase domain-swapped oligomers were detected, including two tetramers, one exchanging only the N-termini, the other being either N- or C-swapped. The C-swapping event, confirmed by results on a BS-K113N mutant, has been firstly seen in BS-RNase here, and probably stabilizes also multimers larger than tetramers. Interestingly, all BS-RNase oligomers are more enzymatically active than the native dimer and, above all, they display a cytotoxic activity that definitely increases with the molecular weight of the multimers. This latter feature, to date unknown for BS-RNase, suggests again that the self-association of RNases strongly modulates their biological and potentially therapeutic properties.