Physiological and molecular characterization of Fe acquisition by tomato plants from natural Fe complexes

Tomasi, N.; De Nobili, M.; Gottardi, S.; Zanin, L.; Mimmo, T.; Varanini, Zeno; Römheld, V.; Pinton, R.; Cesco, S.

doi:10.1007/s00374-012-0706-1

The aim of this work is to evaluate the capability of tomato plants to use different Fe sources, such as Fe citrate, Fe phytosiderophores, and Fe complexed by a water-extractable humic substances (Fe-WEHS) also in re- lation to physiological and molecular adaptations induced by these complexes at the root level. Tomato plants acquired higher amounts of Fe from Fe-WEHS than from the other two sources and this phenomenon occurred only when the treatment lasted 24 h. The higher acquisition of Fe from Fe- WEHS than other sources depended on a reductive mecha- nism and on rhizosphere acidification and appeared to be due neither to a higher apoplastic loading nor to a higher resistance of WEHS to microbial degradation. Supply of the different Fe complexes to deficient plants induced a tran- sient upregulation of Fe(III)-chelate reductase (LeFRO1) and Fe transporter genes, LeIRT1 and LeIRT2. In Fe- WEHS-fed plants, where a quicker and higher upregulation of these genes was evident, a coordination in the expression of LeFRO1, LeIRT1, and LeIRT2 genes occurred already after 1 h treatment when the amount of Fe acquired by the plants from the three sources was similar. Iron from Fe- WEHS could be efficiently acquired in a mixture of natural Fe complexes possibly occurring in the rhizosphere. This phenomenon is due to an altered expression of Fe uptake- related genes and to the root capacity to create favorable conditions for the micronutrient uptake into the rhizosphere.

Titolo:	Physiological and molecular characterization of Fe acquisition by tomato plants from natural Fe complexes
Autori:	Tomasi N. De Nobili M. Gottardi S. Zanin L. Mimmo t. VARANINI, Zeno Römheld V. Pinton R. Cesco S. mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2013
Rivista:	BIOLOGY AND FERTILITY OF SOILS
Abstract:	The aim of this work is to evaluate the capability of tomato plants to use different Fe sources, such as Fe citrate, Fe phytosiderophores, and Fe complexed by a water-extractable humic substances (Fe-WEHS) also in re- lation to physiological and molecular adaptations induced by these complexes at the root level. Tomato plants acquired higher amounts of Fe from Fe-WEHS than from the other two sources and this phenomenon occurred only when the treatment lasted 24 h. The higher acquisition of Fe from Fe- WEHS than other sources depended on a reductive mecha- nism and on rhizosphere acidification and appeared to be due neither to a higher apoplastic loading nor to a higher resistance of WEHS to microbial degradation. Supply of the different Fe complexes to deficient plants induced a tran- sient upregulation of Fe(III)-chelate reductase (LeFRO1) and Fe transporter genes, LeIRT1 and LeIRT2. In Fe- WEHS-fed plants, where a quicker and higher upregulation of these genes was evident, a coordination in the expression of LeFRO1, LeIRT1, and LeIRT2 genes occurred already after 1 h treatment when the amount of Fe acquired by the plants from the three sources was similar. Iron from Fe- WEHS could be efficiently acquired in a mixture of natural Fe complexes possibly occurring in the rhizosphere. This phenomenon is due to an altered expression of Fe uptake- related genes and to the root capacity to create favorable conditions for the micronutrient uptake into the rhizosphere.
Handle:	http://hdl.handle.net/11562/430289
Appare nelle tipologie:	01.01 Articolo in Rivista

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